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Retinoblastoma 1 antibody (RB1) (AA 793-797)

Details for Product anti-RB1 Antibody No. ABIN362687, Supplier: Log in to see
Antigen
  • OSRC
  • P105-RB
  • p105-Rb
  • PP110
  • pp110
  • pRb
  • RB
  • Rb
  • Rb-1
  • RB1
  • RB11
  • zgc:154147
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 793-797
53
50
49
40
35
28
26
19
18
16
16
15
13
9
8
8
7
7
7
6
6
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
525
281
238
11
8
7
5
4
4
3
3
2
2
2
Host
Rabbit
449
79
2
Clonality
Polyclonal
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
22
21
21
16
13
13
10
10
7
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
320
241
173
89
80
73
45
26
22
12
10
8
6
5
2
2
2
1
1
Supplier
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Immunogen Peptide sequence around AA 793-797 (P-S-S-P-L) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Rb protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Rb (RB1 Antibody Abstract)
Background Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Molecular Weight 110 kDa
NCBI Accession NP_000312
UniProt P06400
Research Area Cancer, Transcription Factors
Pathways Cell Division Cycle
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Roesch, Becker, Meyer, Hafner, Wild, Landthaler, Vogt: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Chadee, Kyriakis: "MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation." in: Nature cell biology, Vol. 6, Issue 8, pp. 770-6, 2004 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

Knudsen, Wang: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, 1996 (PubMed).