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Retinoblastoma 1 antibody (RB1) (AA 805-809)

Details for Product anti-RB1 Antibody No. ABIN362690, Supplier: Login to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 805-809
54
52
49
41
36
28
26
21
17
17
16
13
9
8
8
8
7
7
7
6
6
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
526
253
229
11
8
7
5
5
4
4
3
3
2
2
Host
Rabbit
467
68
3
Clonality
Polyclonal
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
22
20
20
15
13
13
10
9
9
7
7
7
7
7
7
Application
Immunohistochemistry (IHC), Western Blotting (WB)
322
215
184
83
74
73
45
35
15
13
10
8
6
5
2
2
2
1
1
Supplier
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Immunogen Peptide sequence around AA 805-809 (Y-I-S-P-L) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Rb protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Rb (RB1 Antibody Abstract)
Background Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter
Molecular Weight 110 kDa
NCBI Accession NP_000312
UniProt P06400
Research Area Cancer, Transcription Factors
Pathways Cell Division Cycle
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Xu, Yan, Williams et al.: "MS4a4B, a CD20 homologue in T cells, inhibits T cell propagation by modulation of cell cycle." in: PLoS ONE, Vol. 5, Issue 11, pp. e13780, 2010 (PubMed).

Background publications Roesch, Becker, Meyer et al.: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Chadee, Kyriakis: "MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation." in: Nature cell biology, Vol. 6, Issue 8, pp. 770-6, 2004 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

Knudsen, Wang: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, 1996 (PubMed).