CDC25C antibody (AA 214-218)

Catalog No. ABIN363151

Product Details anti-CDC25C Antibody

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Binding Specificity: AA 214-218

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: The antibody detects endogenous level of total cdc25C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 214-218 (S-P-S-M-P) derived from Human cdc25C. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: cdc25C (CDC25C Products)

Synonyms: CDC25C antibody, cdc25 antibody, xcdc25c antibody, CDC25 antibody, PPP1R60 antibody, Cdc25 antibody, cdc25-3 antibody, xcdc25-3 antibody, cell division cycle 25C antibody, cell division cycle 25C S homeolog antibody, CDC25C antibody, cdc25c antibody, Cdc25c antibody, cdc25c.S antibody

Background: Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Molecular Weight: 60 kDa

NCBI Accession: NP_001781

UniProt: P30307

Pathways: Cell Division Cycle, M Phase