Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody

Details for Product No. ABIN371878
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Antigen
Epitope
AA 1-238, N-Term
(25), (18), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Aequorea victoria
(341), (31), (11), (4), (3), (3), (2), (1), (1)
Host
Mouse
(148), (146), (56), (26), (4), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(300), (190), (107), (62), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Pubmed 3 references available
Catalog no. ABIN371878
Quantity 50 µL
Price
308.00 $   Plus shipping costs $45.00
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Immunogen Recombinant GFP (1-238 aa) purified from E. coli
Clone AT2G5
Isotype IgG2a
Characteristics Synonyms: Green fluorescent protein, GFP-Tag
Purification Protein-G affinity chromatography
Alternative Name GFP
Background GFP, also known as green fluorescent protein, is a protein produced by the jellyfish(Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where itfunctions as a fluorescent protein tag. It has been expressed in most known cell types andis used as a noninvasive fluorescent marker in living cells and organisms. This proteinenables a wide range of applications where it has functioned as a cell lineage tracer,reporter of gene expression, or as a measure of protein-protein interactions.
UniProt P42212
Research Area Tags/Labels
Application Notes ELISA. Western blot (1: 1,000 - 1: 2,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09% sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody
anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (2) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (Image 2)
Background publications Prasher, Eckenrode, Ward et al.: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Phillips: "Green fluorescent protein--a bright idea for the study of bacterial protein localization." in: FEMS microbiology letters, Vol. 204, Issue 1, pp. 9-18, 2001 (PubMed).

Hosts (148), (146), (56), (26), (4), (2), (1)
Reactivities (341), (31), (11), (4), (3), (3), (2), (1), (1)
Applications (300), (190), (107), (62), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Conjugates (24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (25), (18), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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