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GFP antibody (Green Fluorescent Protein) (AA 1-238)

Details for Product anti-GFP Antibody No. ABIN371878, Supplier: Login to see
Antigen
Epitope
AA 1-238, N-Term
24
18
9
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Aequorea victoria
496
5
4
3
2
2
2
1
1
Host
Mouse
217
157
79
38
11
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
29
28
20
15
13
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
412
235
138
121
106
41
40
35
27
26
15
14
10
9
8
4
3
2
1
1
1
1
1
Supplier
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Immunogen Recombinant GFP (1-238 aa) purified from E. coli
Clone AT2G5
Isotype IgG2a
Characteristics Synonyms: Green fluorescent protein, GFP-Tag
Purification Protein-G affinity chromatography
Alternative Name GFP
Target Type Tag
Background GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Synonyms: GFP-Tag, Green fluorescent protein
UniProt P42212
Research Area Tags/Labels
Pathways
Application Notes ELISA. Western blot (1: 1,000 - 1: 2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (AA 1-238) (ABIN371878) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (AA 1-238) (ABIN371878) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (Image 2)
Background publications Phillips: "Green fluorescent protein--a bright idea for the study of bacterial protein localization." in: FEMS microbiology letters, Vol. 204, Issue 1, pp. 9-18, 2001 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Prasher, Eckenrode, Ward et al.: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).