Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term), (AA 683-712) antibody

Details for Product No. ABIN392233
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms IRAK, pelle, Plpk, mPLK, IRAK-1, Il1rak, IRAK1-S, AA408924, RGD1563841, IRAK1
Epitope
C-Term, AA 683-712
(31), (25), (23), (22), (8), (8), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(175), (93), (77), (36), (24), (24), (12)
Host
Rabbit
(163), (36), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(125), (97), (50), (44), (36), (35), (21), (16), (16), (3), (2), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN392233
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683-712 AA from the C-terminal region of human IRAK.
Clone RB02337
Isotype Ig
Specificity This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683~712 amino acids from the C-terminal region of human IRAK1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name IRAK
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.
Synonyms: interleukin-1 receptor-associated kinase 1
Molecular Weight 76536 DA
Gene ID 3654
UniProt P51617
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term), (AA 683-712) antibody Western blot analysis of anti-IRAK1 Pab (ABIN392233) in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term), (AA 683-712) antibody (2) Western blot analysis of IRAK1 (arrow) using rabbit polyclonal IRAK Antibody (C-term) (ABIN392233). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the IRAK1 gene (Lane 2) (Origene Technologies).
anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term), (AA 683-712) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).

Jiang, Johnson, Nie et al.: "Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 10952-6, 2003 (PubMed).

Jensen, Whitehead: "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1500-6, 2003 (PubMed).

Hosts (163), (36), (1)
Reactivities (175), (93), (77), (36), (24), (24), (12)
Applications (125), (97), (50), (44), (36), (35), (21), (16), (16), (3), (2), (1), (1), (1)
Conjugates (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (31), (25), (23), (22), (8), (8), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top