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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Internal Region)

Details for Product anti-AKT1 Antibody No. ABIN401484, Supplier: Log in to see
Antigen
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
Internal Region
186
136
90
88
54
39
38
37
26
25
21
20
18
16
16
15
15
14
13
13
11
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8
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6
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5
5
5
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4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
1138
528
395
42
41
34
33
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Sheep
889
290
13
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
44
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
954
546
431
215
129
119
112
71
56
35
24
12
11
6
3
3
3
3
1
1
1
1
1
1
Supplier
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Immunogen Synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling
Isotype IgG
Specificity This antibody reacts to AKT.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Mouse, Chicken.
Species reactivity (tested):Human.
Purification Affinity chromatography
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA: 1/5,000 - 1/20,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.9 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Internal Region) (ABIN401484) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region) antibody
Background publications Senthilkumar, Thirumurugan: "GSTM1 and GSTT1 Allele Frequencies among Various Indian and non-Indian Ethnic Groups." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 12, pp. 6263-7, 2013 (PubMed).

Pino, Alata, Dedonder, Jouvet, Sakota, Sekiya: "Photon induced isomerization in the first excited state of the 7-azaindole-(H2O)3 cluster." in: Physical chemistry chemical physics : PCCP, Vol. 13, Issue 13, pp. 6325-31, 2011 (PubMed).

Chu, Li, Singh, Bhatia: "BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia." in: Cancer research, Vol. 67, Issue 14, pp. 7045-53, 2007 (PubMed).

Liu, Zhou, Qian, Shi, Fang, Jiang: "AKT1 amplification regulates cisplatin resistance in human lung cancer cells through the mammalian target of rapamycin/p70S6K1 pathway." in: Cancer research, Vol. 67, Issue 13, pp. 6325-32, 2007 (PubMed).

Dan, Adli, Baldwin: "Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha." in: Cancer research, Vol. 67, Issue 13, pp. 6263-9, 2007 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 (PubMed).

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