Recombinant RFP antibody (AZDye 568)
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- Target See all RFP Antibodies
- RFP (Red Fluorescent Protein (RFP))
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Discosoma
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- This RFP antibody is conjugated to AZDye 568
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Purpose
- Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 2B12
- Specificity
- Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
- Cross-Reactivity (Details)
- Does not cross-react with GFP or mTagBFP derivatives.
- Characteristics
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- Purification
- Produced in: E.coli
- Immunogen
- Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)
- Clone
- 2B12
- Labeling Ratio
- a single fluorophore is coupled to exactly one sdAb
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- Application Notes
- Recommended dilution 1:500
- Comment
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Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Add 200 µl H2O for reconstitution. Possibly add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! - Concentration
- 5 μM
- Buffer
- 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- Expiry Date
- 12 months
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- Target
- RFP (Red Fluorescent Protein (RFP))
- Alternative Name
- RFP (RFP Products)
- Molecular Weight
- 26 kDa
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