Recombinant RFP antibody (Atto 488)
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- Target See all RFP Antibodies
- RFP (Red Fluorescent Protein (RFP))
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Discosoma
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- This RFP antibody is conjugated to Atto 488
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Purpose
- Camelid sdAb anti-RFP conjugated with Atto488, Clones 2B12/2A1
- Specificity
- Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
- Cross-Reactivity (Details)
- Does not cross-react with GFP or mTagBFP derivatives.
- Characteristics
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- Purification
- Produced in: E.coli
- Immunogen
- RFP
- Clone
- 2B12-2A1
- Labeling Ratio
- A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
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- Application Notes
- Recommended dilution 1:500
- Comment
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A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 5 μM
- Buffer
- 1.25 μM of each fluorescently labeled sdAb clone in buffered saline, 50 % glycerol, 0.09 % sodium azide. 5 μM total fluorophore concentration
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light!
- Storage
- -20 °C,-80 °C
- Storage Comment
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- Expiry Date
- 12 months
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- Target
- RFP (Red Fluorescent Protein (RFP))
- Alternative Name
- RFP (RFP Products)
- Molecular Weight
- 26 kDa
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