PAK1 antibody (pThr402, pThr423)

Catalog No. ABIN498797

Product Details anti-PAK1 Antibody

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Binding Specificity: pThr402, pThr423

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of p-PAK1/2 protein, only when phosphorylated at Thr423/402.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1 (PAK1 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, PAKalpha antibody, AW045634 antibody, PAK-1 antibody, Paka antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, p21 protein (Cdc42/Rac)-activated kinase 1 antibody, Serine/threonine-protein kinase pak-1 antibody, pak1.S antibody, PAK1 antibody, Pak1 antibody, pak-1 antibody

Background: Three isoforms of Serine/Threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. The site of autophosphorylation on αPAK is Thr 423, which is in the kinase activation loop. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK-4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac 1 and Cdc42, they do not interact with Rho.Synonyms: Alpha-PAK, Gamma-PAK, PAK 1, PAK 2, PAK alpha, PAK gamma, PAK-1, PAK-2, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, p21-activated kinase 1, p21-activated kinase 2, p58, p65-PAK

Molecular Weight: approx. 60 kDa

Gene ID: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis