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Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Intermediate Domain 2)

Details for Product anti-CASP9 Antibody No. ABIN499579, Supplier: Log in to see
Antigen
  • CASP9
  • AI115399
  • APAF-3
  • AW493809
  • CASP-9
  • Caspase-9
  • ICE-LAP6
  • Mch6
  • Apaf3
  • Casp-9-CTD
  • Casp9_v1
  • Ice-Lap6
  • APAF3
  • MCH6
  • PPP1R56
  • caspase 9
  • caspase 9, apoptosis-related cysteine peptidase
  • caspase-9-like
  • CASP9
  • LOC100101592
  • Casp9
  • LOC100518913
Alternatives
anti-Human Caspase 9 antibody for Western Blotting
Epitope
Intermediate Domain 2
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Reactivity
Human
427
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
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Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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Options
Supplier
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Immunogen Human Caspase-9 (Intermediate Domain 2) Peptide
Isotype IgG
Specificity Caspase-9 antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9. It has no cross response to other members in caspase family.
Purification Affinity chromatography purified via peptide column
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Gene ID 842
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA. Western Blot: Caspase-9 antibody can be used for detection of caspase-9 at 1/1000 to1/2000 dilution. A 46 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Intermediate Domain 2) (ABIN499579) Immunocytochemistry staining of K562 cells using AP30206PU-N Caspase-9 antibody at 2 ...
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Intermediate Domain 2) (ABIN499579) Western blot analysis of caspase-9 in HeLa whole cell lysate with AP30206PU-N Caspase...
Background publications Duan, Orth, Chinnaiyan, Poirier, Froelich, He, Dixit: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996 (PubMed).