TANK-Binding Kinase 1 (TBK1) (C-Term) antibody

Details for Product No. ABIN500308
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Antigen
Synonyms nak, t2k, TBK1, wu:fk70c05, zgc:136548, NAK, T2K, 1200008B05Rik, AI462036, AW048562
Epitope
C-Term
(20), (14), (14), (13), (7), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(81), (44), (25), (15), (14), (12), (2)
Host
Rabbit
(85), (10), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(68), (39), (20), (16), (15), (10), (7), (7), (4), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN500308
401.50 $
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Immunogen Peptide from C-term of human NAK / TBK1 (Cat. No. 2351P)
Isotype IgG
Specificity This antibody detects TBK1 at C-term. It has no cross response to IKKa, IKKb, IKKy, or IKKe.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Alternative Name TBK1
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. Phosphorylation of I-kappaB by IkappaB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-kappaB. The IKK complex contains IKKalpha, IKKbeta, and IKKgamma. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kappaB-activating kinase), and T2K (1-3). NAK/TBK1 activates IKKbeta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kappaB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1,2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).Synonyms: NAK, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1
Gene ID 29110
NCBI Accession NP_037386
UniProt Q9UHD2
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL, an 84 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Background publications Pomerantz, Baltimore: "NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase." in: The EMBO journal, Vol. 18, Issue 23, pp. 6694-704, 2000 (PubMed).

Tojima, Fujimoto, Delhase et al.: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000 (PubMed).

Bonnard, Mirtsos, Suzuki et al.: "Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-kappaB-dependent gene transcription." in: The EMBO journal, Vol. 19, Issue 18, pp. 4976-85, 2000 (PubMed).

Rao, Kadesch: "The intracellular form of notch blocks transforming growth factor beta-mediated growth arrest in Mv1Lu epithelial cells." in: Molecular and cellular biology, Vol. 23, Issue 18, pp. 6694-701, 2003 (PubMed).

Angelone, Quintieri, Amodio et al.: "Endocrine orchestration of cardiovascular, gastrointestinal and hypothalamic control." in: Current medicinal chemistry, Vol. 18, Issue 32, pp. 4976-86, 2011 (PubMed).

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