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NOXA antibody (Phorbol-12-Myristate-13-Acetate-Induced Protein 1) (N-Term)

Details for Product anti-PMAIP1 Antibody No. ABIN500377, Supplier: Log in to see
Antigen
  • APR
  • Noxa
  • NOXA
  • PMAIP1
  • TN3
  • zgc:162379
Alternatives
anti-Human NOXA antibody for Immunocytochemistry
Epitope
N-Term
18
7
5
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
98
77
40
Host
Rabbit
48
43
27
17
Clonality
Polyclonal
Conjugate
This NOXA antibody is un-conjugated
6
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
129
39
33
30
22
21
1
1
1
Supplier
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Immunogen Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.
Isotype IgG
Specificity This antibody detects NOXA.
Purification Peptide affinity chromatography
Alternative Name NOXA (PMAIP1 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified , which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 . Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).Synonyms: PMA-induced protein 1, PMAIP1, Phorbol-12-myristate-13-acetate-induced protein 1
Gene ID 58801
NCBI Accession NP_067426
UniProt Q9JM54
Pathways p53 Signaling
Application Notes ELISA. Western blot. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0,5 mg/mL
Buffer PBS containing 0.02 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NOXA antibody (Phorbol-12-Myristate-13-Acetate-Induced Protein 1) (N-Term) (ABIN500377) Immunohistochemistry of Noxa in human stomach tissue with this product at 1 μg/ml.
Western Blotting (WB) image for anti-NOXA antibody (Phorbol-12-Myristate-13-Acetate-Induced Protein 1) (N-Term) (ABIN500377) Western blot analysis of Noxa in human stomach tissue lysate with this product at (A)...
Background publications Eferl, Ricci, Kenner, Zenz, David, Rath, Wagner: "Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53." in: Cell, Vol. 112, Issue 2, pp. 181-92, 2003 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Yu, Zhang, Hwang, Kinzler, Vogelstein: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Oda, Ohki, Murasawa, Nemoto, Shibue, Yamashita, Tokino, Taniguchi, Tanaka: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).