PDK2 antibody (AA 187-276)

Catalog No. ABIN562160

The Mouse Monoclonal anti-PDK2 antibody has been validated for WB, ELISA, IHC (p) and RNAi. It is suitable to detect PDK2 in samples from Human. There are 4+ publications available.

Quick Overview for PDK2 antibody (AA 187-276) (ABIN562160)

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Clone: 2G1

Product Details anti-PDK2 Antibody

Binding Specificity: AA 187-276

Purpose: Mouse monoclonal antibody raised against a partial recombinant PDK2.

Sequence: HIGSIDPNCN VSEVVKDAYD MAKLLCDKYY MASPDLEIQE INAANSKQPI HMVYVPSHLY HMLFELFKNA MRATVESHES SLILPPIKVM

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-PDK2 antibody (ABIN562160)

Gannon, Ables, Crawford, Lowe, Offield, Magnuson, Wright: "pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis." in: Developmental biology, Vol. 314, Issue 2, pp. 406-17, (2008) (PubMed).

Babu, Deering, Mirmira: "A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis." in: Molecular genetics and metabolism, Vol. 92, Issue 1-2, pp. 43-55, (2007) (PubMed).

Gu, Dubauskaite, Melton: "Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors." in: Development (Cambridge, England), Vol. 129, Issue 10, pp. 2447-57, (2002) (PubMed).

Ohlsson, Karlsson, Edlund: "IPF1, a homeodomain-containing transactivator of the insulin gene." in: The EMBO journal, Vol. 12, Issue 11, pp. 4251-9, (1993) (PubMed).

Target Details for PDK2

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Alternative Name: PDK2

Background: Full Gene Name: pyruvate dehydrogenase kinase, isozyme 2
Synonyms: PDHK2

Gene ID: 5164

Pathways: PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect