Zyxin (ZYX) (full length) antibody

Details for Product No. ABIN563471
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Antigen
Synonyms BcDNA:LD06023, CG32018, DLim-1, DmLIM-1, Dmel\\CG32018, Lim102EF, ZYX102, Zyx102, Zyx102EF, lim, zyx102, zyxin, zgc:171680, ZYX, zyx, MGC145270, ESP-2, HED-2, 9530098H06Rik, R75157
Epitope
full length
(19), (12), (7), (7), (6), (4), (3), (2), (2), (1), (1)
Reactivity
Human
(78), (24), (21), (2), (1), (1)
Host
Mouse
(52), (15), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB), Immunoprecipitation (IP), RNA Interference (RNAi)
(69), (36), (14), (13), (11), (10), (10), (4), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Availability Will be delivered in 11 to 12 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN563471
330.00 $
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Immunogen ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 kDa.
Immunogen sequence: MAAPRPSPAI SVSVSAPAFY APQEKFGPVV APKPKVNPFR PGDSEPPPAP GAQRAQMGRV GEIPPPPPED FPLPPPPLAG DGDDAEGALG GAFPPPPPPI EESFPPAPLE EEIFPSPPPP PEEEGGPEAP IPPPPQPREK VSSIDLEIDS LSSLLDDMTK NDPFKARVSS GYVPPPVATP FSSKSSTKPA AGGTAPLPPW KSPSSSQPLP QVPAPAQSQT QFHVQPQPQP KPQVQLHVQS QTQPVSLANT QPRGPPASSP APAPKFSPVT PKFTPVASKF SPGAPGGSGS QPNQKLGHPE ALSAGTGSPQ PPSFTYAQQR EKPRVQEKQH PVPPPAQNQN QVRSPGAPGP LTLKEVEELE QLTQQLMQDM EHPQRQNVAV NELCGRCHQP LARAQPAVRA LGQLFHIACF TCHQCAQQLQ GQQFYSLEGA PYCEGCYTDT LEKCNTCGEP ITDRMLRATG KAYHPHCFTC VVCARPLEGT SFIVDQANRP HCVPDYHKQY APRCSVCSEP IMPEPGRDET VRVVALDKNF HMKCYECEDC GKPLSIEADD NGCFPLDGHV LCRKCHTARA QT
Clone 2C10-4A7
Isotype IgG1 kappa
Alternative Name ZYX
Background Gene: zyxin
Synonyms: ESP-2, HED-2
Gene ID 7791
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20
Storage Comment Store at -20
Supplier Images
anti-Zyxin (ZYX) (full length) antibody Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.

Lane 1: ZYX transfected lysate(61.3 KDa).
Lane 2: Non-transfected lysate.
anti-Zyxin (ZYX) (full length) antibody (2) Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # H00007791-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
anti-Zyxin (ZYX) (full length) antibody (3) Immunofluorescence of monoclonal antibody to ZYX on HeLa cell. (antibody concentration 10 ᄉg/mL)
anti-Zyxin (ZYX) (full length) antibody (4) Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead
anti-Zyxin (ZYX) (full length) antibody (5) Detection limit for recombinant GST tagged ZYX is 1 ng/mL as a capture antibody.
anti-Zyxin (ZYX) (full length) antibody (6) Western Blot detection against Immunogen (88.66 KDa) .
Background publications Hirata, Tatsumi, Sokabe: "Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner." in: Journal of cell science, Vol. 121, Issue Pt 17, pp. 2795-804, 2008 (PubMed).

Validation Images
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