POLG antibody (AA 829-1201)
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- Target See all POLG Antibodies
- POLG (Polymerase (DNA Directed), gamma (POLG))
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Binding Specificity
- AA 829-1201
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLG antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Specificity
- This antibody detects POLG1.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Immunoaffinity Chromatography
- Immunogen
- Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma.
- Top Product
- Discover our top product POLG Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.7 mg/mL
- Buffer
- PBS, pH 7, 1 % BSA, 20 % Glycerol and 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody (in aliquots) at -20 °C. Dilute only prior to immediate use.
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- Target
- POLG (Polymerase (DNA Directed), gamma (POLG))
- Alternative Name
- POLG1 (POLG Products)
- Synonyms
- POLG antibody, MDP1 antibody, MIRAS antibody, MTDPS4A antibody, MTDPS4B antibody, PEO antibody, POLG1 antibody, POLGA antibody, SANDO antibody, SCAE antibody, AA409516 antibody, PolgA antibody, polymerase (DNA) gamma, catalytic subunit L homeolog antibody, DNA polymerase gamma, catalytic subunit antibody, polymerase (DNA directed), gamma antibody, polg.L antibody, Polg antibody, POLG antibody, polg antibody
- Target Type
- Viral Protein
- Background
- As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil DNA glycosylase and apurinic / apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic / apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.Synonyms: DNA polymerase subunit gamma-1, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLGA, PolG-alpha
- Gene ID
- 5428
- NCBI Accession
- NP_001119603
- UniProt
- P54098
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