BRA-4F1 produces mouse IgM immunoglobulins specific for the Lewis x antigen. It is used in immunohistochemistry and flow cytometry on studies of activated T cells, analysis of myeloid leukemias, myeloid differentiation and identification of Reed-Sternberg cells in subtypes of Hodgkin’s disease
Antigen distribution: CD15 is expressed on neutrophils, eosinophils and monocytes, but not on platelets, erythrocytes, normal B and T cells. It is also present in embryonic tissues and adenocarcinomas, myeloid leukemias and Reed-Sternberg cells [1,2]. CD15 antibodies recognize the terminal trisaccharide structure which is also referred to as the Lewis x antigen. This structure is found on a variety of glycoproteins and glycolipids at the cell surface [3,4]. Cellular Expression: Granulocytes, monocytes.