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Retinoblastoma 1 antibody (RB1) (AA 858-886)

Details for Product anti-RB1 Antibody No. ABIN652516, Supplier: Login to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 858-886, C-Term
53
52
49
41
36
28
26
21
17
17
16
13
9
8
8
8
7
7
7
6
6
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
526
254
230
11
8
7
5
5
4
4
3
3
2
2
Host
Rabbit
467
68
3
Clonality (Clone)
Polyclonal ()
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
22
20
20
15
13
13
10
9
9
7
7
7
7
7
7
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
322
216
184
83
73
73
45
35
15
13
10
8
5
5
2
2
2
1
1
Supplier
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Immunogen This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 AA from the C-terminal region of human RB1.
Clone RB20715
Isotype Ig
Specificity This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 865~894 amino acids from the C-terminal region of human RB1
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name RB1 (RB1 Antibody Abstract)
Background RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Synonyms: Retinoblastoma-associated protein, pRb, Rb, pp110, p105-Rb, RB1
Molecular Weight 106159 DA
Gene ID 5925
UniProt P06400
Research Area Cancer, Transcription Factors, Signaling, Neurology, Cell Structure
Pathways Cell Division Cycle
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Retinoblastoma 1 antibody (RB1) (AA 858-886) (ABIN652516) Western blot analysis of RB1 Antibody (C-term) (ABIN652516) in 293 cell line lysates ...
Immunohistochemistry (IHC) image for anti-Retinoblastoma 1 antibody (RB1) (AA 858-886) (ABIN652516) Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody (C...
Flow Cytometry (FACS) image for anti-Retinoblastoma 1 antibody (RB1) (AA 858-886) (ABIN652516) RB1 Antibody (C-term) (ABIN652516) flow cytometric analysis of 293 cells (bottom hist...
Background publications Connell-Crowley, Harper, Goodrich: "Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 287-301, 1997 (PubMed).

Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).