Glutaminase antibody (GLS) (AA 516-545)

Details for Product anti-GLS Antibody No. ABIN652757, Supplier: Log in to see
Antigen
  • GA
  • PAG
  • AI314027
  • 6330442B14
  • B230365M23Rik
  • gls
  • si:dz87i4.2
  • wu:fa97e05
  • CG8772
  • CG8872
  • Dmel\\CG42708
  • Dmel_CG8772
  • BA3155
  • AAD20
  • GAC
  • GAM
  • GLS1
  • KGA
  • Glut
  • RATGLUT
  • A330074B06Rik
  • AI195532
  • GLS
  • Lga
  • glutaminase
  • glutaminase b
  • CG42708 gene product from transcript CG42708-RA
  • hypothetical protein
  • Glutaminase
  • glutaminase 2 (liver, mitochondrial)
  • GLS
  • Gls
  • gls
  • glsb
  • CG42708
  • glutaminase
  • glsA-2
  • Arnit_3113
  • Celal_2913
  • Odosp_0379
  • Odosp_1505
  • Celly_0289
  • Weevi_2101
  • Mesop_4683
  • Gls2
Alternatives
anti-Mouse (Murine) Glutaminase antibody for ELISA
Epitope
AA 516-545, C-Term
20
10
9
8
6
2
2
1
Reactivity
Human, Mouse (Murine)
75
45
32
4
2
2
2
2
1
1
Host
Rabbit
62
20
Clonality (Clone)
Polyclonal ()
Conjugate
This Glutaminase antibody is un-conjugated
5
5
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
65
43
26
19
14
13
11
9
5
2
1
Options
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Immunogen This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 AA from the C-terminal region of human GLS.
Clone RB22875
Isotype Ig
Predicted Reactivity Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name GLS (GLS Antibody Abstract)
Background Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Synonyms: K-glutaminase, L-glutamine amidohydrolase, Glutaminase kidney isoform, mitochondrial, GLS, GLS, GLS1, KIAA0838
Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Nitrogen metabolism, Proximal tubule bicarbonate reclamation
Molecular Weight 65,460 Da, Isoform 3 Da
Gene ID 2744
UniProt O94925
Research Area Neurology
Pathways Feeding Behaviour, Dicarboxylic Acid Transport
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Glutaminase antibody (GLS) (AA 516-545) (ABIN652757) Western blot analysis of GLS Antibody (C-term) (ABIN652757) in mouse liver tissue lys...
Immunohistochemistry (IHC) image for anti-Glutaminase antibody (GLS) (AA 516-545) (ABIN652757) GLS Antibody (C-term) (ABIN652757) IHC analysis in formalin fixed and paraffin embedd...
Flow Cytometry (FACS) image for anti-Glutaminase antibody (GLS) (AA 516-545) (ABIN652757) GLS Antibody (C-term) (ABIN652757) flow cytometric analysis of HepG2 cells (right his...
Background publications Swierczyński, Bereznowski, Makarewicz: "Phosphate-dependent glutaminase of rat skeletal muscle. Some properties and possible role in glutamine metabolism." in: Biochimica et biophysica acta, Vol. 1157, Issue 1, pp. 55-62, 1993 (PubMed).