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V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3)
Clonality Monoclonal
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN659026
Quantity 0.1 mg  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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Additional Information
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Alternative name AKT3
Gene ID 10000
UniProt Q9Y243, NP_859029.1
Immunogen This AKT3 monoclonal antibody is generated from mouse immunized with AKT3 recombinant protein.
Isotype Ig
Description Other names: STK-2, Protein kinase B gamma, PKB gamma, Protein kinase Akt-3, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase,PKBG, AKT3
Characteristics Purified Mouse Monoclonal Antibody (Mab)
Specificity This AKT3 monoclonal antibody is generated from mouse immunized with AKT3 recombinant protein.
Molecular Weight 55775 DA
Comments

Background: AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin, Signaling
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Yang, Wang, Chan et al.: "The E3 ligase TRAF6 regulates Akt ubiquitination and activation." in: Science (New York, N.Y.), Vol. 325, Issue 5944, pp. 1134-8, 2009 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3)", type "Antibodies"
Hosts Rabbit (68), Mouse (19), Goat (7), Chicken (1)
Reactivities Human (94), Mouse (Murine) (38), Rat (Rattus) (34), Cow (Bovine) (26), Dog (Canine) (26), Chicken (24), Pig (Porcine) (23), Sheep (Ovine) (23), Cat (Feline) (1)
Applications Western Blotting (WB) (68), ELISA (56), Flow Cytometry (FACS) (23), Immunofluorescence (IF) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (9), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Fluorescence Microscopy (FM) (2), Dot Blot (Dot) (1), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes N-Term (6), pSer472 (6), Internal Region (5), AA 1-479 (2), AA 119-131 (2), AA 119-136 (2), C-Term (2), Ser472 (2), Thr447 (2), Transcript Variant 1 (2), pSer166 (1)