Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) antibody

Antigen: Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1)

Synonyms: RIP, RIP1, FLJ39204, Rinp, Rip1, D330015H01Rik

Clonality: Monoclonal (G322-2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN967308

Additional Information

Alternative name: RIP

Immunogen: truncated RIP fusion protein

Components: 1) 51-6559GR: Purified Mouse Anti-Human RIP.
Quantity: 50 µg (3 ea).
Concentration: 0.25 mg/ml.
Clone: G322-2.
Immunogen: truncated RIP fusion protein.
Isotype: Mouse IgG1.
Molecular Weight: 74 kDa.
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and Less or equal than 0.09% sodium azide.

2) 51-16526N: Jurkat Cell Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).

Format: Liquid

Isotype: IgG1, kappa

Clone: G322-2

Description: RIP (receptor interacting protein) is a 74 kDa serine/threonine kinase which may be recruited to TNFR type 1 and Fas (CD95) receptor signal complexes following ligand binding. RIP interacts with other signal proteins within these complexes (e.g., RAIDD) and has also been shown to interact with pro-caspase-2. RIP contains an N-terminal kinase domain as well as a C-terminal death domain that is homologous to intracellular death domain of Fas. Over expression of RIP in vitro is sufficient to induce cell death, demonstrating that RIP functions as an apoptosis-inducing protein. Interaction of the Fas death domain with other intracellular proteins like RIP is an important step leading to downstream components in apoptotic signaling pathways. Clone G322-2 recognizes human RIP. A recombinant truncated human RIP:tagged fusion protein, lacking the kinase domain of RIP, was used as immunogen. The specificity of the antibody was verified by ELISA, immunoprecipitation and western blot analysis. The antibody is routinely tested by western blot analysis in human Jurkat T cells where it recognizes RIP as a 74 kDa band. Smaller molecular weight breakdown bands of ~30 kDa, 22 kDa, and/or 16 kDa are sometimes observed.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 74 kDa

Comments:

Related Products: ABIN968537

Application Details

Application Notes: Additional control lysate (ABIN968537) is sold separately.

Concentration: 0.25 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of RIP. Lysate from Jurkat cells was probed with anti-RIP (clone G322-2) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). RIP is identified as a band of 74 kDa.

Publications

Product: Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).