Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)

Synonyms: MEK1, MKK1, MAPKK1, PRKMK1, Mek1, MEKK1, Prkmk1

Binding Site: pSer298

Clonality: Monoclonal (J114-64)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967599

Additional Information

Alternative name: MEK1

Immunogen: Phosphorylated Human MEK1 Peptide

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1, kappa

Clone: J114-64

Description: MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 45 kDa

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Signaling, Tyrosine Kinases

Restrictions: For Research Use only

Images

Western blot analysis of MEK1 (pS298) in mouse embryonic fibroblasts. Lysates from detached (trypsinized, lanes 1-3) and attached (80-90% confluent, lanes 4-6) NIH/3T3 cell line were probed with purified mouse anti-MEK1 (pS298) monoclonal antibody at concentrations of 0.015 (lanes 1 and 4), 0.008 (lanes 2 and 5), and 0.004 µg/ml (lanes 3 and 6). MEK1 (pS298) is identified as a band of 45 kDa in the treated cells.

Publications

Product: Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, 2001 (PubMed).

Eblen, Slack, Weber et al.: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, 2002 (PubMed).

Slack-Davis, Eblen, Zecevic et al.: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, 2003 (PubMed).