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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibody

Details for Product No. ABIN967599
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Antigen
Synonyms
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN  ... show more
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN KINASE KINASE, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, NMAPKK, MEK show less
Epitope
pSer298
(31), (27), (25), (24), (24), (21), (21), (19), (19), (12), (12), (11), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(400), (214), (204), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1), (1)
Host
Mouse
(370), (41), (2), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (1), (1)
Application
Western Blotting (WB)
(321), (116), (98), (92), (60), (49), (21), (20), (14), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN967599
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Immunogen Phosphorylated Human MEK1 Peptide
Clone J114-64
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name MEK1
Background MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.
Molecular Weight 45 kDa
Research Area Signaling, Tyrosine Kinases
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibody Western blot analysis of MEK1 (pS298) in mouse embryonic fibroblasts. Lysates from detached (trypsinized, lanes 1-3) and attached (80-90% confluent, lanes 4-6) NIH/3T3 cell line were probed with purified mouse anti-MEK1 (pS298) monoclonal antibody at concentrations of 0.015 (lanes 1 and 4), 0.008 (lanes 2 and 5), and 0.004 µg/ml (lanes 3 and 6). MEK1 (pS298) is identified as a band of 45 kDa in the treated cells.
Product cited in: Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, 2001 (PubMed).

Eblen, Slack, Weber et al.: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, 2002 (PubMed).

Slack-Davis, Eblen, Zecevic et al.: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, 2003 (PubMed).

Validation Images
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