Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody

Details for Product No. ABIN967742
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Synonyms zgc:136695, LCK, Hck-3, Lsk, Lskt, p56, p56Lck, LSK, YT16, p56lck, pp58lck, P56LCK, tkl, Lck1, Lcktkr
AA 1-191
(58), (48), (23), (16), (12), (12), (12), (11), (11), (11), (11), (9), (8), (7), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(345), (164), (123), (5), (4), (4), (3), (2)
(258), (80), (15)
Clonality (Clone)
Monoclonal ()
(8), (8), (8), (6), (6), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(310), (142), (58), (53), (34), (31), (22), (20), (11), (8), (6), (6), (4), (4), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 50 μg
Shipping to United States ( )
Immunogen Human Lck aa. 1-191
Clone 28-Lck
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Lck (LCK Antibody Abstract)
Background The p56[lck] protein kinase is a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. Within the src family of PTKs, lck, fyn, and Yes are expressed in T cells. The unique N-terminal domain of p56[lck] interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface major histocompatibility complex (MHC) class II and class I molecules, respectively. These complexes interact with the T cell antigen receptor (TCR) in the early stages of T cell activation. In addition, an activated lck kinase increases responsiveness of some T cell hybridomas to antigen. The phosphorylation status and, therefore, the activity of p56[lck] kinase is regulated by the CD45 tyrosine protein phosphatase. Several studies suggest that lck has many functions critical to T cell development and activation. Mice lacking a functional lck gene are drastically impaired in the production of T lymphocytes. Variants of the human Jurkat T cell line that do not express p56[lck] exhibit a diminished response to stimulation of the T cell receptor. Evidence suggests that lck is directly upstream from PI3-kinase in the signal transduction cascade in T cell activation.
Molecular Weight 56 kDa
Research Area Immunology, Tyrosine Kinases, Adaptive Immunity, CD Antigens, Signaling
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody (ABIN967742) Western blot analysis of Lck on jurkat lysate. Lane 1: 1:5000, lane 2: 1:10000, lane ...
Image no. 2 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody (ABIN967742) Jurkat
Image no. 3 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody (ABIN967742) anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody (Image 3)
Western Blotting (WB) image for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody (ABIN967742) anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-191) antibody (Image 4)
Product cited in: Zeyda, Staffler, Horejsi et al.: "LAT displacement from lipid rafts as a molecular mechanism for the inhibition of T cell signaling by polyunsaturated fatty acids." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28418-23, 2002 (PubMed).

Zhou, Magnuson, Cheng et al.: "Hierarchy of protein tyrosine kinases in interleukin-2 (IL-2) signaling: activation of syk depends on Jak3; however, neither Syk nor Lck is required for IL-2-mediated STAT activation." in: Molecular and cellular biology, Vol. 20, Issue 12, pp. 4371-80, 2000 (PubMed).

Maccalli, Pisarra, Vegetti et al.: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000 (PubMed).

Xu, Littman: "A kinase-independent function of Lck in potentiating antigen-specific T cell activation." in: Cell, Vol. 74, Issue 4, pp. 633-43, 1993 (PubMed).

Horak, Gress, Lucas et al.: "T-lymphocyte interleukin 2-dependent tyrosine protein kinase signal transduction involves the activation of p56lck." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 5, pp. 1996-2000, 1991 (PubMed).

Catalog No. ABIN967742
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