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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (AA 1-197)

Details for Product anti-CDKN1B Antibody No. ABIN967850, Supplier: Log in to see
Antigen
  • CDKN1B
  • CIB
  • CIBP
  • KIP1
  • PRKDCIP
  • SIP2-28
  • p27Kip1
  • AA408329
  • AI843786
  • Kip1
  • p27
  • CDKN4
  • Cdki1b
  • P27KIP1
  • MEN1B
  • MEN4
  • cdkn1bl
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • FAM14D
  • ISG12
  • ISG12A
  • P27
  • 1500011J20Rik
  • 2610202L11Rik
  • Bridge-1
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • calcium and integrin binding 1 (calmyrin)
  • cyclin kinase inhibitor p30 Kip1
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • cyclin-dependent kinase inhibitor 1Bb
  • interferon, alpha-inducible protein 27
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
  • CDKN1B
  • CIB1
  • LOC100689401
  • Cdkn1b
  • cdkn1ba
  • cdkn1bb
  • IFI27
  • Psmd9
Alternatives
anti-Human CDKN1B antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 1-197
95
56
51
44
34
27
27
22
21
21
21
19
19
17
16
15
13
13
9
8
5
5
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
702
355
293
91
44
28
20
10
9
9
3
3
3
2
1
1
1
Host
Mouse
484
213
38
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CDKN1B antibody is un-conjugated
34
33
32
31
25
25
8
8
6
6
5
5
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
534
328
198
174
152
108
98
97
53
37
28
14
10
3
3
1
1
1
Options
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Immunogen Mouse Kip1 aa. 1-197
Clone 57-Kip1-p27
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Chicken, Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p27 Kip1 (CDKN1B Antibody Abstract)
Background Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFbeta-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-beta in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-beta also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-beta treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro. This antibody is routinely tested by western blot analysis.
Molecular Weight 27 kDa
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (AA 1-197) (ABIN967850) Western blot analysis of p27[Kip1] on HeLa cell lysate. Lane 1: 1:2500, lane 2: 1:500...
Immunofluorescence (IF) image for anti-CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (AA 1-197) (ABIN967850) Immunofluorescent staining of HeLa cells.
Product cited in: Guo, Packard, Krolewski, Harris, Manglapus, Schwob: "Expression of pax6 and sox2 in adult olfactory epithelium." in: The Journal of comparative neurology, Vol. 518, Issue 21, pp. 4395-418, 2010 (PubMed).

Barnouin, Dubuisson, Child, Fernandez de Mattos, Glassford, Medema, Mann, Lam: "H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13761-70, 2002 (PubMed).

Williamson, Dadmanesh, Koeffler: "BRCA1 transactivates the cyclin-dependent kinase inhibitor p27(Kip1)." in: Oncogene, Vol. 21, Issue 20, pp. 3199-206, 2002 (PubMed).

Frey, Clark, Leontieva, Uronis, Black, Black: "Protein kinase C signaling mediates a program of cell cycle withdrawal in the intestinal epithelium." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 763-78, 2000 (PubMed).

Polyak, Kato, Solomon, Sherr, Massague, Roberts, Koff: "p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest." in: Genes & development, Vol. 8, Issue 1, pp. 9-22, 1994 (PubMed).

Background publications Polyak, Lee, Erdjument-Bromage, Koff, Roberts, Tempst, Massagué: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).