Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 385-650) antibody

Antigen: Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1)

Synonyms: RIP, RIP1, FLJ39204, Rinp, Rip1, D330015H01Rik

Binding Site: AA 385-650

Clonality: Monoclonal (38)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN967985

Additional Information

Alternative name: RIP

Immunogen: Human RIP

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Format: Liquid

Isotype: IgG2a

Clone: 38

Description: Binding or cross linking of the Fas antigen (also known as APO-1 and CD95) is known to elicit apoptosis in susceptible cells. Fas is a member of a family of cell surface receptors which includes tumor necrosis factor receptors (TNF-R, and TNF-R2) and nerve growth factor receptors (NGF-R), CD40, OX40, CD30, CD27, and 4-1BB. Several members of this family have been shown to regulate or induce cell death (TNF-R1 and TNF-R2). A 74 kDa member of this family protein named RIP (Receptor Interacting Protein) contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic death domain present in both Fas and TNF-R1. Both Fas and RIP have been shown to require this death domain to induce apoptosis and overexpression of RIP has been shown to induce cell death in transfected cells.
Synonyms: Receptor Interacting Protein

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 74 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of RIP on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-RIP antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).

Publications

Product: Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).

Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).

Lewis, Devin, Miller et al.: "Disruption of hsp90 function results in degradation of the death domain kinase, receptor-interacting protein (RIP), and blockage of tumor necrosis factor-induced nuclear factor-kappaB activation." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10519-26, 2000 (PubMed).

Fulda, Meyer, Debatin: "Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression." in: Cancer research, Vol. 60, Issue 14, pp. 3947-56, 2000 (PubMed).

Devin, Lin, Yamaoka et al.: "The alpha and beta subunits of IkappaB kinase (IKK) mediate TRAF2-dependent IKK recruitment to tumor necrosis factor (TNF) receptor 1 in response to TNF." in: Molecular and cellular biology, Vol. 21, Issue 12, pp. 3986-94, 2001 (PubMed).