Proliferating Cell Nuclear Antigen (PCNA) (AA 68-230) antibody

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyms: MGC8367, PCNAR, Pcna/cyclin, pcna

Binding Site: AA 68-230

Clonality: Monoclonal (24)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968096

Additional Information

Alternative name: PCNA

Immunogen: Human PCNA

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 24

Description: Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins, 2) synthesis and degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase delta. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
Synonyms: Proliferating Cell Nuclear Antigen

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 36 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer, Cell Cycle, Chromatin Binding Proteins, DNA/RNA, Proliferation Markers

Restrictions: For Research Use only

Images

Western blot analysis of PCNA on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-PCNA antibody.

Immunofluorescence staining of AN3 CA cells (Human endometrial adenocarcinoma, ATCC HTB-111).

Publications

Product: Travali, Ku, Rizzo et al.: "Structure of the human gene for the proliferating cell nuclear antigen." in: The Journal of biological chemistry, Vol. 264, Issue 13, pp. 7466-72, 1989 (PubMed).

Moore, Scheinman, Bellgrau: "The identification of a novel T cell activation state controlled by a diabetogenic gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 241-8, 2001 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Li, Dowbenko, Lasky: "AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 11352-61, 2002 (PubMed).

Reilly, Wysocka, Herr: "Inactivation of the retinoblastoma protein family can bypass the HCF-1 defect in tsBN67 cell proliferation and cytokinesis." in: Molecular and cellular biology, Vol. 22, Issue 19, pp. 6767-78, 2002 (PubMed).