Proliferating Cell Nuclear Antigen (PCNA) (AA 68-230) antibody

Details for Product No. ABIN968096
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Antigen
Synonyms
53/13, CG9193, DmPCNA, Dmel\\CG9193, MUS209, Mus209, PCNA, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, pcna, AO090003000846, PCNAR, Pcna/cyclin, cb16, fb36g03, wu:fa28e03, wu:fb ... show more
53/13, CG9193, DmPCNA, Dmel\\CG9193, MUS209, Mus209, PCNA, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, pcna, AO090003000846, PCNAR, Pcna/cyclin, cb16, fb36g03, wu:fa28e03, wu:fb36g03, pcna-A show less
Epitope
AA 68-230
(26), (17), (15), (13), (6), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(321), (182), (136), (79), (67), (62), (13), (13), (12), (12), (11), (7), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(189), (185), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(19), (19), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(252), (126), (83), (69), (63), (60), (55), (37), (24), (21), (12), (6), (4), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 50 μg
Options
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Catalog No. ABIN968096
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Immunogen Human PCNA
Clone 24
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PCNA
Background Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins, 2) synthesis and degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase delta. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
Synonyms: Proliferating Cell Nuclear Antigen
Molecular Weight 36 kDa
Research Area Cancer, Cell Cycle, Chromatin Binding Proteins, DNA/RNA, Proliferation Markers
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 68-230) antibody Immunofluorescence staining of AN3 CA cells (Human endometrial adenocarcinoma, ATCC HTB-111).
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 68-230) antibody (2) Western blot analysis of PCNA on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-PCNA antibody.
Product cited in: Travali, Ku, Rizzo et al.: "Structure of the human gene for the proliferating cell nuclear antigen." in: The Journal of biological chemistry, Vol. 264, Issue 13, pp. 7466-72, 1989 (PubMed).

Moore, Scheinman, Bellgrau: "The identification of a novel T cell activation state controlled by a diabetogenic gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 241-8, 2001 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Li, Dowbenko, Lasky: "AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 11352-61, 2002 (PubMed).

Reilly, Wysocka, Herr: "Inactivation of the retinoblastoma protein family can bypass the HCF-1 defect in tsBN67 cell proliferation and cytokinesis." in: Molecular and cellular biology, Vol. 22, Issue 19, pp. 6767-78, 2002 (PubMed).

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