Protein KINase Family Member (Kin-1) (KIN-1) (AA 18-347) antibody

Details for Product No. ABIN968293
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Antigen
Epitope
AA 18-347
(1), (1)
Reactivity
Human
(1), (1)
Host
Mouse
(1), (1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
(2)
Pubmed 5 references available
Quantity 150 μg
Options
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Catalog No. ABIN968293
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Immunogen Human PKA[Calpha] subunit
Clone 5B
Isotype IgG2b
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PKA C
Background CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. The levels of expression of the different subunits vary according to cell and tissue type.
Molecular Weight 40 kDa
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Protein KINase Family Member (Kin-1) (KIN-1) (AA 18-347) antibody Immunofluorescent staining of HeLa cells.
anti-Protein KINase Family Member (Kin-1) (KIN-1) (AA 18-347) antibody (2) Western blot analysis of PKA[C] on HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of antiPKA[C].
anti-Protein KINase Family Member (Kin-1) (KIN-1) (AA 18-347) antibody (3) anti-Protein KINase Family Member (Kin-1) (KIN-1) (AA 18-347) antibody (Image 3)
Product cited in: Taylor, Buechler, Yonemoto: "cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes." in: Annual review of biochemistry, Vol. 59, pp. 971-1005, 1990 (PubMed).

Orellana, Marfella-Scivittaro: "Distinctive cyclic AMP-dependent protein kinase subunit localization is associated with cyst formation and loss of tubulogenic capacity in Madin-Darby canine kidney cell clones." in: The Journal of biological chemistry, Vol. 275, Issue 28, pp. 21233-40, 2000 (PubMed).

Westphal, Soderling, Alto et al.: "Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold." in: The EMBO journal, Vol. 19, Issue 17, pp. 4589-600, 2000 (PubMed).

Chen, Yu, Lee et al.: "CREB is one component of the binding complex of the Ces-2/E2A-HLF binding element and is an integral part of the interleukin-3 survival signal." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4636-46, 2001 (PubMed).

DeSouza, Reiken, Ondrias et al.: "Protein kinase A and two phosphatases are components of the inositol 1,4,5-trisphosphate receptor macromolecular signaling complex." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39397-400, 2002 (PubMed).

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