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anti-Mouse (Murine) CD11b antibody for Blocking Reagent

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Mouse (Murine)
611
460
142
67
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Rat
477
410
113
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
163
123
87
51
18
17
12
12
11
11
11
10
9
9
7
6
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
797
242
205
159
135
111
78
74
72
67
19
14
11
7
6
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Catalog No. ABIN1177001
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN2688906 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8
0.0008696448 ABIN438560 BR Neut Rat IgG2b Log in to see AP-MAB0813
0.0008696448 ABIN1967641 BR FACS FITC Rat IgG2b Log in to see mCD11b
0.0008696448 ABIN3024273 BR FACS IF Rat IgG2b, kappa Log in to see M1-70
0.0008696448 ABIN2688907 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70
0.0008696448 ABIN3024274 BR FACS IF Rat IgG2b, kappa Log in to see M1-70
0.0008696448 ABIN2742255 BR FACS IP ELISA Rat IgG2b, kappa Log in to see M1-70

Top referenced anti-CD11b antibody for Blocking Reagent

Similar anti-CD11b Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 8 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 389 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunohistochemistry (IHC) 107 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 76 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 127 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine)
(611), (460), (142), (67), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Rat
(477), (410), (113), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(163), (123), (87), (51), (18), (17), (12), (12), (11), (11), (11), (10), (9), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(797), (242), (205), (159), (135), (111), (78), (74), (72), (67), (19), (14), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN1177001) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse Splenic Cells
Clone M1-70
Isotype IgG2b, kappa

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN1177001) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background The M1/70 antibody reacts with the 170-kDa alpha[M] chain of Mac-1 (CD11b/CD18, alpha[M]beta[2] integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported.
Synonyms: CR-3 alpha chain, Itgam, Integrin alpha M, Ly-40, Mac-1 alpha
Research Area Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN1177001) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN1177001) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD11b Antibody (ABIN1177001)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, 1996

Kishimoto, Jutila, Berg, Butcher: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." in: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, 1989

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978