anti-Mouse (Murine) CD11b antibody for Functional Studies

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human, Mouse (Murine)
732
514
192
79
31
19
17
15
13
11
10
9
8
7
5
4
4
4
4
2
Host
Rat
533
409
168
17
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
150
128
94
56
24
24
20
17
15
14
14
13
13
13
11
11
11
10
9
7
7
6
6
6
6
6
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
930
237
235
172
172
137
100
98
97
67
25
21
21
18
14
8
5
5
4
3
3
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN1450195
$ 544.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6
1 ABIN1690733 FACS Func IHC (f) IP APC Rat IgG2b Log in to see M1-70 10
1 ABIN457402 FACS Func IHC (f) IP Rat IgG2b Log in to see M1-70 5
1 ABIN490173 BR FACS Func IHC (fro) IP Neut Rat IgG2b Log in to see M1-70
1 ABIN457346 FACS Func IHC (f) IP FITC Rat IgG2b Log in to see M1-70 8
1 ABIN457369 FACS Func IHC (f) IP PE Rat IgG2b Log in to see M1-70 9
1 ABIN2478233 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 4
1 ABIN3200842 FACS Func IHC (fro) IP FITC Rat IgG2b Log in to see M1-70
1 ABIN609287 Func IHC (fro) FACS IP Rat IgG2b Log in to see M1-70-15
1 ABIN3199418 FACS Func IHC (fro) IP APC Rat IgG2b Log in to see M1-70
1 ABIN3200843 FACS Func IHC (fro) IP PE Rat IgG2b Log in to see M1-70
1 ABIN1820254 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15
1 ABIN2742255 BR ELISA FACS Func IF IHC (fro) IP Rat IgG2b, kappa Log in to see M1-70
1 ABIN3199417 FACS Func IHC (fro) IP Rat IgG2b Log in to see M1-70
1 ABIN1820248 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6
1 ABIN2567136 Func FACS IF IP IHC Rat IgG2b kappa Log in to see M1-70
1 ABIN2567137 Func FACS IHC Rat IgG2b kappa Log in to see M1-70
1 ABIN5633268 FACS Func IHC (f) IP Rat IgG2b Log in to see M1-70
1 ABIN3036618 Func FACS IP IHC APC Rat IgG2b Log in to see

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine)
(732), (514), (192), (79), (31), (19), (17), (15), (13), (11), (10), (9), (8), (7), (5), (4), (4), (4), (4), (2)
Host Rat
(533), (409), (168), (17), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(150), (128), (94), (56), (24), (24), (20), (17), (15), (14), (14), (13), (13), (13), (11), (11), (11), (10), (9), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
(930), (237), (235), (172), (172), (137), (100), (98), (97), (67), (25), (21), (21), (18), (14), (8), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
Supplier Log in to see

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1450195) Images
Purification Affinity Chromatography on Protein G
Endotoxin Level < 0.01EU/ug
Immunogen Thioglycollate-elicted Peritoneal Macrophages (TPM). Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line.
Clone 5C6
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN1450195) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10090
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN1450195) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN1450195) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate Buffered Saline, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-CD11b antibody (ABIN1450195)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Tysseling, Mithal, Sahni, Birch, Jung, Belmadani, Miller, Kessler: "SDF1 in the dorsal corticospinal tract promotes CXCR4+ cell migration after spinal cord injury." in: Journal of neuroinflammation, Vol. 8, pp. 16, 2011

Wu, Wu, Wang, Wang: "Expression and cellular localization of cyclooxygenases and prostaglandin E synthases in the hemorrhagic brain." in: Journal of neuroinflammation, Vol. 8, pp. 22, 2011

Terwel, Steffensen, Verghese, Kummer, Gustafsson, Holtzman, Heneka: "Critical role of astroglial apolipoprotein E and liver X receptor-α expression for microglial Aβ phagocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 19, pp. 7049-59, 2011

McDonald, Cortini, Rosas, Fossati-Jimack, Ling, Lewis, Dewitt, Liddiard, Brown, Jones, Hallett, Botto, Taylor: "In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 1972-82, 2011

Devey, Ferenbach, Mohr, Sangster, Bellamy, Hughes, Wigmore: "Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 17, Issue 1, pp. 65-72, 2008

Basso, Frenkel, Quintana, Costa-Pinto, Petrovic-Stojkovic, Puckett, Monsonego, Bar-Shir, Engel, Gozin, Weiner: "Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis." in: The Journal of clinical investigation, Vol. 118, Issue 4, pp. 1532-43, 2008

Khorooshi, Babcock, Owens: "NF-kappaB-driven STAT2 and CCL2 expression in astrocytes in response to brain injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 10, pp. 7284-91, 2008

Hickman, Allison, El Khoury: "Microglial dysfunction and defective beta-amyloid clearance pathways in aging Alzheimer's disease mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 33, pp. 8354-60, 2008

Clausen, Lambertsen, Babcock, Holm, Dagnaes-Hansen, Finsen: "Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice." in: Journal of neuroinflammation, Vol. 5, pp. 46, 2008

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008

Rosen, Milon, Gordon: "Antibody to the murine type 3 complement receptor inhibits T lymphocyte-dependent recruitment of myelomonocytic cells in vivo." in: The Journal of experimental medicine, Vol. 169, Issue 2, pp. 535-48, 1989

Rosen, Gordon: "Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo." in: The Journal of experimental medicine, Vol. 166, Issue 6, pp. 1685-701, 1987