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anti-Mouse (Murine) CD11b antibody for Intracellular Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Mouse (Murine)
551
430
133
48
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Rat
447
389
103
16
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to PE/Dazzle™ 594
162
120
85
51
18
17
12
11
11
8
7
7
7
7
6
6
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
758
219
177
132
108
82
75
67
48
38
19
15
11
7
5
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Catalog No. ABIN2659637
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008719615 ABIN2660477 ICFC PerCP-Cy5.5 Rat IgG2b kappa Log in to see M1-70 11
0.0008719615 ABIN2659638 ICFC PE/Dazzle™ 594 Rat IgG2b kappa Log in to see M1-70 11
0.0008719615 ABIN2660478 ICFC PerCP-Cy5.5 Rat IgG2b kappa Log in to see M1-70 11

Top referenced anti-CD11b antibody for Intracellular Flow Cytometry

Similar anti-CD11b Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 89 Antibodies 38 Antibodies
Staining Methods (StM) 2 Antibodies 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 6 Antibodies 4 Antibodies
In vitro Assay (in vitro) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 101 Antibodies 108 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 93 Antibodies 82 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 53 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine)
(551), (430), (133), (48), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Rat
(447), (389), (103), (16), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to PE/Dazzle™ 594
(162), (120), (85), (51), (18), (17), (12), (11), (11), (8), (7), (7), (7), (7), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(758), (219), (177), (132), (108), (82), (75), (67), (48), (38), (19), (15), (11), (7), (5), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2659637) Images
Cross-Reactivity Cynomolgus, Rhesus Monkey, Baboon, Chimpanzee, Rabbit
Purification The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Clone M1-70
Isotype IgG2b kappa

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN2659637) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN2659637) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN2659637) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD11b Antibody (ABIN2659637)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003

DAmico, Wu: "The early progenitors of mouse dendritic cells and plasmacytoid predendritic cells are within the bone marrow hemopoietic precursors expressing Flt3." in: The Journal of experimental medicine, Vol. 198, Issue 2, pp. 293-303, 2003

Coxon, Rieu, Barkalow, Askari, Sharpe, von Andrian, Arnaout, Mayadas: "A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation." in: Immunity, Vol. 5, Issue 6, pp. 653-66, 1997

Allen, Aderem: "Molecular definition of distinct cytoskeletal structures involved in complement- and Fc receptor-mediated phagocytosis in macrophages." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 627-37, 1996

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994

Noel, Mosser, Edelson: "Role of complement in mouse macrophage binding of Haemophilus influenzae type b." in: The Journal of clinical investigation, Vol. 85, Issue 1, pp. 208-18, 1990

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983

Ho, Springer: "Biosynthesis and assembly of the alpha and beta subunits of Mac-1, a macrophage glycoprotein associated with complement receptor function." in: The Journal of biological chemistry, Vol. 258, Issue 5, pp. 2766-9, 1983

Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2659637) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M)  (PE/Dazzle™ 594) (ABIN2659637) anti-Integrin alpha M (ITGAM) antibody (PE/Dazzle™ 594)