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anti-Mouse (Murine) CD11b antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Mouse (Murine)
552
430
133
48
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Rat
447
389
103
16
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
162
120
85
51
18
17
12
11
11
8
7
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
757
219
177
131
108
82
75
67
48
38
19
15
11
6
6
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Catalog No. ABIN2688908
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Top referenced anti-CD11b antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-CD11b Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 8 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 361 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 53 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 108 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Mouse (Murine)
(552), (430), (133), (48), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Rat
(447), (389), (103), (16), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(162), (120), (85), (51), (18), (17), (12), (11), (11), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(757), (219), (177), (131), (108), (82), (75), (67), (48), (38), (19), (15), (11), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2688908) Images
Brand BD Pharmingen™
Characteristics The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170- kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. Immunohistochemical staining of CD11b positive cells. An acetone-fixed, frozen section of normal mouse spleen was stained with the Purified Rat Anti-Mouse CD11b antibody. CD11b positive cells can be identified by the intense brown labeling of cell surface membranes (20X magnification).

BD Pharmingen™ Purified Rat Anti-Mouse CD11b - Purified - Clone M1/70 - Isotype Rat IgG2b, κ - Reactivity Ms - 1 mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse Splenic Cells
Clone M1-70
Isotype IgG2b, kappa

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN2688908) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Synonyms: Itgam, Integrin alpha-M, Ly-40, Mac-1a, Mac-1 alpha, CR3A, CR-3 alpha chain
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN2688908) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN2688908) Images back to top
Format Liquid
Concentration 125 μg/mL
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD11b Antibody (ABIN2688908)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Leenen, de Bruijn, Voerman, Campbell, van Ewijk: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, 1994

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, 1992

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979