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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||93 Antibodies||41 Antibodies||28 Antibodies|
|Staining Methods (StM)||2 Antibodies||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Multiplex Assay (MA)||1 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||6 Antibodies||4 Antibodies|
|In vitro Assay (in vitro)||1 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||101 Antibodies||108 Antibodies||28 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies||36 Antibodies||14 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Epitope||AA 17-382 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Integrin alpha-M(ITGAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: integrin, alpha M (complement component 3 receptor 3 subunit)
Protein Name: Integrin alpha-M
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.|
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|Alternative Name||ITGAM (ITGAM Antibody Abstract)|
|Background||Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.|
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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