anti-Rabbit CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human, Mouse (Murine), Rabbit
732
514
192
78
31
19
17
15
13
11
10
9
8
7
5
4
4
4
4
2
Host
Rat
533
409
168
17
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to PE
150
127
94
56
24
24
20
17
15
14
14
13
13
13
11
11
11
10
9
7
7
6
6
6
6
6
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS)
930
238
235
173
172
138
100
98
97
68
25
21
21
18
14
8
5
5
4
3
3
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN118147
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.996978 ABIN118142 FACS FITC Rat IgG2b Log in to see M1-70-15 7
13.996978 ABIN118143 FACS FITC Rat IgG2b Log in to see M1-70-15 6
13.996978 ABIN2478237 FACS PE Rat IgG2b Log in to see M1-70-15 4
13.996978 ABIN2478196 IHC (fro) FACS IF IP Mouse IgG1 Log in to see 198 4
13.996978 ABIN4289459 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 488 Rat IgG2b Log in to see M1-70-15
13.996978 ABIN1690733 FACS Func IHC (f) IP APC Rat IgG2b Log in to see M1-70 10
1 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
1 ABIN573960 IHC (fro) FACS IHC (p) IP Rat IgG2b Log in to see M1-70-15
1 ABIN118144 FACS FITC Rat IgG2b Log in to see M1-70-15 6
1 ABIN319552 FACS FITC Rat IgG2b Log in to see M1-70-15 6
1 ABIN319551 FACS Biotin Rat IgG2b Log in to see M1-70-15 6
1 ABIN319554 FACS PE Rat IgG2b Log in to see M1-70-15 6
1 ABIN319555 FACS PE Rat IgG2b Log in to see M1-70-15 6
1 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
1 ABIN2478219 IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN118141 FACS PE-Cy5 Rat IgG2b Log in to see M1-70-15 7
1 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6
1 ABIN2478225 FACS Biotin Rat IgG2b Log in to see M1-70-15 4
1 ABIN2478230 FACS FITC Rat IgG2b Log in to see M1-70-15 4

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine), Rabbit
(732), (514), (192), (78), (31), (19), (17), (15), (13), (11), (10), (9), (8), (7), (5), (4), (4), (4), (4), (2)
Host Rat
(533), (409), (168), (17), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to PE
(150), (127), (94), (56), (24), (24), (20), (17), (15), (14), (14), (13), (13), (13), (11), (11), (11), (10), (9), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(930), (238), (235), (173), (172), (138), (100), (98), (97), (68), (25), (21), (21), (18), (14), (8), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN118147) Images
Purification Affinity Chromatography on Protein G
Immunogen T cell enriched splenocytes from B10 mice. Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Clone M1-70-15
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN118147) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10090
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN118147) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN118147) Images back to top
Reconstitution Restore with distilled water.
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

References for anti-CD11b antibody (ABIN118147)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Klingel, Schnorr, Sauter, Szalay, Kandolf: "beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis." in: The American journal of pathology, Vol. 162, Issue 5, pp. 1709-20, 2003

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN118147) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (PE) (ABIN118147) Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophag...