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|Application / Reactivity||Human||Mouse (Murine)||Rabbit|
|Western Blotting (WB)||93 Antibodies||41 Antibodies||2 Antibodies|
|Staining Methods (StM)||2 Antibodies||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Multiplex Assay (MA)||1 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||6 Antibodies||4 Antibodies|
|In vitro Assay (in vitro)||1 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||101 Antibodies||108 Antibodies||22 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||2 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies||36 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Reactivity||Human, Mouse (Murine), Rabbit Alternatives|
|Conjugate||This CD11b antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
|6 references available|
|Supplier||Log in to see|
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118143) Images
|Purification||Affinity chromatography on Protein A|
|Immunogen||T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN118143) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
|Background||MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor|
|Research Area||Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN118143) Images back to top
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN118143) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD11b Antibody (ABIN118143)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Klingel, Schnorr, Sauter et al.: "beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis." in: The American journal of pathology, Vol. 162, Issue 5, pp. 1709-20, 2003 (PubMed).
Welt, Edelman, Simon et al.: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000 (PubMed).
Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).
Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).
Springer, Galfré, Secher et al.: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).
Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118143) back to top