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anti-Rabbit CD11b antibody for Functional Studies

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human, Mouse (Murine), Rabbit
643
485
198
64
26
20
10
9
9
8
8
5
4
3
2
2
2
1
1
Host
Rat
494
393
157
18
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
166
128
94
51
16
15
15
15
14
13
11
11
9
9
6
6
6
6
6
5
5
5
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
843
231
204
175
149
148
104
100
93
70
22
19
17
13
8
6
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN319553
$ 544.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN457402 FACS Func IHC (f) IP Rat IgG2b Log in to see M1-70 5
1 ABIN457346 FACS Func IHC (f) IP FITC Rat IgG2b Log in to see M1-70 8
1 ABIN457369 FACS Func IHC (f) IP PE Rat IgG2b Log in to see M1-70 9
1 ABIN1690733 FACS Func IHC (f) IP APC Rat IgG2b Log in to see M1-70 10
1 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN3199418 FACS Func IHC (fro) IP APC Rat IgG2b Log in to see M1-70
1 ABIN3200842 FACS Func IHC (fro) IP FITC Rat IgG2b Log in to see M1-70
1 ABIN3200843 FACS Func IHC (fro) IP PE Rat IgG2b Log in to see M1-70
1 ABIN3199417 FACS Func IHC (fro) IP Rat IgG2b Log in to see M1-70
1 ABIN3036617 Func FACS IP IHC PE Rat IgG2b Log in to see
1 ABIN3036618 Func FACS IP IHC APC Rat IgG2b Log in to see
1 ABIN3036619 Func FACS IP IHC FITC Rat IgG2b Log in to see
1 ABIN3036611 Func FACS IP IHC Rat IgG2b Log in to see

Top referenced anti-CD11b antibody for Functional Studies

Similar anti-CD11b Antibodies

Application / Reactivity Rabbit Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 7 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 62 Antibodies
Functional Studies (Func) 14 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Western Blotting (WB) 2 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
ELISA 13 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine), Rabbit
(643), (485), (198), (64), (26), (20), (10), (9), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1)
Host Rat
(494), (393), (157), (18), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(166), (128), (94), (51), (16), (15), (15), (15), (14), (13), (11), (11), (9), (9), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(843), (231), (204), (175), (149), (148), (104), (100), (93), (70), (22), (19), (17), (13), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN319553) Images
Cross-Reactivity (Details) Species reactivity (expected):Human, Rabbit.
Species reactivity (tested):Mouse.
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Endotoxin Level Low Endotoxin
Immunogen T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Clone M1-70-15
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN319553) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10090
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN319553) Images back to top
Application Notes Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Immunoprecipitation. Flow Cytometry: 1/50 - 1/100 (routinely tested in flow cytometry on mouse peritoneal macrophages). Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level < 0.01EU/ug
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN319553) Images back to top
Concentration 1.0 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-CD11b Antibody (ABIN319553)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Klingel, Schnorr, Sauter, Szalay, Kandolf: "beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis." in: The American journal of pathology, Vol. 162, Issue 5, pp. 1709-20, 2003

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978