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anti-Mouse (Murine) CFLAR antibody for Immunohistochemistry (Frozen Sections)

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • 2310024N18Rik
  • A430105C05Rik
  • c-Flip
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • CASH
  • Cash
  • CASP8AP1
  • Casper
  • cflar
  • CFLAR
  • CLARP
  • Clarp1
  • ENSMUSG00000072980
  • FLAME
  • FLAME-1
  • FLAME1
  • Flip
  • FLIP
  • Gm9845
  • I-FLICE
  • MRIT
Epitope
C-Term, AA 191-209
36
21
19
13
13
13
10
8
6
6
6
6
6
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
199
78
57
2
2
2
2
2
1
Host
Rabbit
155
60
4
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
7
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
179
73
48
36
34
22
22
15
13
8
3
2
1
1
1
Supplier
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Catalog No. ABIN318973
$ 423.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN1858388 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 210-460 Log in to see Polyclonal

Top referenced anti-CFLAR antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CFLAR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 26 Antibodies 66 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 4 Antibodies 13 Antibodies
Immunocytochemistry (ICC) 13 Antibodies 30 Antibodies
Immunofluorescence (IF) 7 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 2 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 57 Antibodies
ELISA (Capture) 1 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope C-Term, AA 191-209
(36), (21), (19), (13), (13), (13), (10), (8), (6), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(199), (78), (57), (2), (2), (2), (2), (2), (1)
Host Rabbit
(155), (60), (4)
Clonality
Polyclonal
Conjugate This CFLAR antibody is un-conjugated
(7), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(179), (73), (48), (36), (34), (22), (22), (15), (13), (8), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN318973) Images
Specificity This antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).
Purification Purified
Immunogen A peptide corresponding to aa191 - 209 at the C-terminus of human FLIP delta/FLIPs form.
Isotype IgG

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR Antibody (ABIN318973) Images back to top
Antigen
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants. FLIP is widely expressed in human tissues and a diverse range of cell lines.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
NCBI Accession NP_001120655
UniProt O15519
Pathways Apoptosis

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR Antibody (ABIN318973) Images back to top
Application Notes Immunohistochemistry on frozen sections: 2g/mL. Western Blot: 1: 1000, detects bands of approximately 35 kDa and 25 kDa in Jurkat celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR Antibody (ABIN318973) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CFLAR Antibody (ABIN318973)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Background publications

Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997

Hu, Vincenz, Ni, Gentz, Dixit: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997

Irmler, Thome, Hahne, Schneider, Hofmann, Steiner, Bodmer, Schröter, Burns, Mattmann, Rimoldi, French, Tschopp: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997

Images

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Supplier Images
 image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN318973) anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209), (C-Term) antibody
 image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN318973) anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209), (C-Term) antibody (Image 2)