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anti-Dog (Canine) Cytochrome C antibody for Immunofluorescence

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • bln
  • bs30f07.y1
  • CG13263
  • CG17903
  • CYC
  • cyc
  • CYCS
  • cycs
  • CYCSA
  • cyct
  • cyt-c-d
  • cyt-c-p
  • Cyt-c1
  • cyt-c2
  • Cyt-c2
  • cyt.c
  • Cyt C
  • Cyt c
  • cyt c
  • Cytc
  • cytc
  • CytC-1
  • CytC-2
  • Cytc-d
  • cyt c-d
  • Cytc-p
  • cyt c-p
  • CytC1
  • cytochrome c
  • DC3
  • dc3
  • dc4
  • DC4
  • DMc01
  • Dmel\\CG13263
  • Dmel\\CG17903
  • ENSMUSG00000058927
  • hcs
  • HCS
  • ms(2)04445
  • pDMc01
  • pDMc02
  • THC4
  • wu:fk52b01
  • zgc:86706
Epitope
AA 66-104, AA 93-104
18
13
11
8
6
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
182
109
100
47
23
16
16
11
10
8
7
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
137
113
7
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
16
9
4
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
179
67
61
47
43
41
33
17
14
11
11
6
6
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN3024593
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850475 ABIN3025122 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 1-80 Log in to see 7H8-2C12
0.0010850475 ABIN3024590 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05
0.0010850475 ABIN3024594 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 66-104, AA 93-104 Log in to see SPM389
0.0010850475 ABIN3025124 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 1-80 Log in to see 7H8-2C12
0.0010850475 ABIN3024592 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05

Similar anti-Cytochrome C Antibodies

Application / Reactivity Pigeon Dog (Canine) Horse (Equine) Human Mouse (Murine) Rat (Rattus) Fruit Fly (Drosophila melanogaster) Frog
Western Blotting (WB) 20 Antibodies 12 Antibodies 36 Antibodies 122 Antibodies 69 Antibodies 75 Antibodies 10 Antibodies 6 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 3 Antibodies 2 Antibodies 2 Antibodies
Intracellular Staining (ICS) 4 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 23 Antibodies

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 66-104, AA 93-104
(18), (13), (11), (8), (6), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
(182), (109), (100), (47), (23), (16), (16), (11), (10), (8), (7), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(137), (113), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(16), (9), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(179), (67), (61), (47), (43), (41), (33), (17), (14), (11), (11), (6), (6), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Purity Protein G affinity chromatography
Immunogen Amino acids 66-104 of pigeon Cytochrome C were used as the immunogen for the Cytochrome C antibody. It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.
Clone SPM389
Isotype IgG2b, kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Cyt°Chrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).