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anti-Fruit Fly (Drosophila melanogaster) Cytochrome C antibody for Western Blotting

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • bln
  • bs30f07.y1
  • CG13263
  • CG17903
  • CYC
  • cyc
  • CYCS
  • cycs
  • CYCSA
  • cyct
  • cyt-c-d
  • cyt-c-p
  • Cyt-c1
  • cyt-c2
  • Cyt-c2
  • cyt.c
  • Cyt C
  • Cyt c
  • cyt c
  • Cytc
  • cytc
  • CytC-1
  • CytC-2
  • Cytc-d
  • cyt c-d
  • Cytc-p
  • cyt c-p
  • CytC1
  • cytochrome c
  • DC3
  • dc3
  • dc4
  • DC4
  • DMc01
  • Dmel\\CG13263
  • Dmel\\CG17903
  • ENSMUSG00000058927
  • hcs
  • HCS
  • ms(2)04445
  • pDMc01
  • pDMc02
  • THC4
  • wu:fk52b01
  • zgc:86706
Epitope
AA 1-80, AA 93-104
18
13
11
8
6
6
6
4
4
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
183
110
101
48
24
17
16
11
11
8
8
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
137
114
7
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is conjugated to Biotin
16
9
4
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
180
67
61
48
44
41
34
17
14
11
11
6
6
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN251788
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN1723142 ICC IHC (p) WB Mouse IgG2b Log in to see 7H8-2C12
0.0011806369 ABIN1002181 IP WB Rabbit Log in to see Polyclonal
0.0011806369 ABIN1724341 FACS ICC IHC (p) WB Mouse IgG2a Log in to see 7H8-2C12
0.0011806369 ABIN3024593 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 66-104, AA 93-104 Log in to see SPM389
0.0011806369 ABIN3025122 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 1-80 Log in to see 7H8-2C12
0.0011806369 ABIN3024590 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05
0.0011806369 ABIN3024594 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 66-104, AA 93-104 Log in to see SPM389
0.0011806369 ABIN3025124 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 1-80 Log in to see 7H8-2C12
0.0011806369 ABIN3024592 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05

Similar anti-Cytochrome C Antibodies

Application / Reactivity Horse (Equine) Human Mouse (Murine) Rat (Rattus) Fruit Fly (Drosophila melanogaster)
Western Blotting (WB) 36 Antibodies 122 Antibodies 69 Antibodies 75 Antibodies 10 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 3 Antibodies 2 Antibodies 2 Antibodies
Intracellular Staining (ICS) 4 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 23 Antibodies 10 Antibodies 17 Antibodies 14 Antibodies 1 Antibodies

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 1-80, AA 93-104
(18), (13), (11), (8), (6), (6), (6), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(183), (110), (101), (48), (24), (17), (16), (11), (11), (8), (8), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(137), (114), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is conjugated to Biotin
(16), (9), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
(180), (67), (61), (48), (44), (41), (34), (17), (14), (11), (11), (6), (6), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Specificity An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Purification Protein G purified
Immunogen Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Clone 7H8-2C12
Isotype IgG2b kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background In addition to its well-known role in electron transport, cytochrome c (CYT) has beenidentified as a mediator of apoptosis or programmed cell death (1). Cytochrome ctranslocates from mitochondria into the cytoplasm in the early phases of apoptosis andplays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3 (2).Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activatecapase-3 (3). Alternate Names: anti-CYC antibody, anti-CYCS antibody, anti-Cytochrome c somatic antibody, anti-HCSantibody.
Gene Symbol: CYCS
Gene ID 54205, 13063, 25309
Research Area Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al.
Recommended dilutions: Immunohistochemistry, Immunohistochemistry-Frozen 1:10-1:500, Western Blot 0.05-0.5 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (AA 1-80) (Biotin) (ABIN251788) Western blot detection of Cytochrome C in 15 mgs of HeLa cell lysate using ABIN251788...