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anti-Pig (Porcine) Cytochrome C antibody for Immunoprecipitation

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • bln
  • bs30f07.y1
  • CG13263
  • CG17903
  • CYC
  • cyc
  • CYCS
  • cycs
  • CYCSA
  • cyct
  • cyt-c-d
  • cyt-c-p
  • Cyt-c1
  • cyt-c2
  • Cyt-c2
  • cyt.c
  • Cyt C
  • Cyt c
  • cyt c
  • Cytc
  • cytc
  • CytC-1
  • CytC-2
  • Cytc-d
  • cyt c-d
  • Cytc-p
  • cyt c-p
  • CytC1
  • cytochrome c
  • DC3
  • dc3
  • dc4
  • DC4
  • DMc01
  • Dmel\\CG13263
  • Dmel\\CG17903
  • ENSMUSG00000058927
  • hcs
  • HCS
  • ms(2)04445
  • pDMc01
  • pDMc02
  • THC4
  • wu:fk52b01
  • zgc:86706
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
306
229
172
94
62
56
28
28
28
24
24
19
16
8
7
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
236
138
7
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
20
14
10
7
7
6
6
5
5
5
5
5
5
5
5
5
5
5
2
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
277
157
138
81
69
67
47
34
19
11
11
8
6
6
5
4
4
4
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN967529
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089564006 ABIN1002181 IP WB Rabbit Log in to see Polyclonal

Top referenced anti-Cytochrome C antibody for Immunoprecipitation

Similar anti-Cytochrome C Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Pig (Porcine)
Western Blotting (WB) 220 Antibodies 117 Antibodies 169 Antibodies 24 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 8 Antibodies 7 Antibodies 7 Antibodies
Intracellular Staining (ICS) 4 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 30 Antibodies 16 Antibodies 23 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 129 Antibodies 69 Antibodies 120 Antibodies 18 Antibodies

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(306), (229), (172), (94), (62), (56), (28), (28), (28), (24), (24), (19), (16), (8), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(236), (138), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(20), (14), (10), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(277), (157), (138), (81), (69), (67), (47), (34), (19), (11), (11), (8), (6), (6), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling References for anti-Cytochrome C Antibody (ABIN967529) Images
Brand BD Pharmingen™
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Horse (Equine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Synthetic Peptides of Pigeon Cytochrome C
Clone 7H8-2C12
Isotype IgG2b, kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C Antibody (ABIN967529) Images back to top
Antigen
Alternative Name Cytochrome c (CYCS Antibody Abstract)
Background A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c, and d) according to spectrochemical characteristics, and there are five cytochromes between Coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble peripheral membrane protein of the mitochondria and transports electrons from the Coenzyme QH2 - Cytochrome c reductase complex to the Cytochrome c oxidase complex. Recently it has been suggested that Cytochrome c may be required for cells to undergo apoptosis. Mitochondria may be involved in apoptosis by releasing Cytochrome c to the cytosol where it functions in the activation of Caspase 3 (CPP32), an ICE family molecule that has been shown to trigger apoptosis when activated. In the cytoplasm Cytochrome C might exist in polymeric forms, appearing as a 58-60 kDa band by western blot analysis, rather than the monomeric form. Monomeric Cytochrome c migrates at a reduced molecular weight of 15 kDa.
Clone 7H8.2C12 recognizes the denatured form of pigeon, horse, rat, mouse and human Cytochrome c. It does not recognize the native form of Cytochrome c. Clone 7H8.2C12 recognizes an epitope within amino acids 93-104 (inclusive) of pigeon Cytochrome C, based on competitive ELISA results. Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon Cytochrome c were used as immunogens. Clone 7H8.2C12 is used for western blot detection of Cytochrome c. Clone 6H2.B4, is used for immunoprecipitation. Cell lines P388D1 mouse lymphoma (ATCC CCL-46), HeLa human carcinoma (ATCC CCL-2), Jurkat T leukemia (ATCC TIB-152), and NIH/3T3 mouse fibroblast cells (ATCC CRL-1658) may be used as positive control cell lysates.
This antibody is routinely tested by western blot analysis.
Research Area Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C Antibody (ABIN967529) Images back to top
Application Notes Applications include western blot analysis (1-2 µg/ml). Clone 7H8.2C12 is not useful for immunoprecipitation. However, clone 6H2.B4 is useful for immunoprecipitation of Cytochrome c.
Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C Antibody (ABIN967529) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.

References for anti-Cytochrome C Antibody (ABIN967529)

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top
Product cited in:

Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996

Goshorn, Retzel, Jemmerson: "Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c." in: The Journal of biological chemistry, Vol. 266, Issue 4, pp. 2134-42, 1991

Background publications

Jemmerson, Johnson, Burrell, Taylor, Jenkins: "A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells." in: European journal of immunology, Vol. 21, Issue 1, pp. 143-51, 1991

Images

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling References for anti-Cytochrome C Antibody (ABIN967529) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (ABIN967529) Western blot analysis of cytochrome c. Lysate from HL-60 cells were probed with anti-...
 image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (ABIN967529) anti-Cytochrome C, Somatic (CYCS) antibody (Image 2)
Western Blotting (WB) image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (ABIN967529) anti-Cytochrome C, Somatic (CYCS) antibody (Image 3)