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anti-Dog (Canine) Perforin 1 antibody for Western Blotting

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • PRF1
  • LOC443187
  • perforin
  • prf1
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1-like
  • PRF1
  • LOC443187
  • prf1
  • Prf1
  • LOC100342396
Epitope
N-Term
20
16
9
6
6
5
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
157
48
23
20
1
1
Host
Rabbit
101
71
21
Clonality
Polyclonal
Conjugate
This Perforin 1 antibody is un-conjugated
16
12
10
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
95
83
44
41
37
37
36
34
30
6
6
6
3
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN2775294
$ 289.00
Plus shipping costs $45.00

Top referenced anti-Perforin 1 antibody for Western Blotting

Similar anti-Perforin 1 Antibodies

Application / Reactivity Human Rat (Rattus) Cow (Bovine) Dog (Canine) Pig (Porcine) Horse (Equine) Guinea Pig Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 1 Antibodies 2 Antibodies
Western Blotting (WB) 20 Antibodies 64 Antibodies 3 Antibodies 1 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 29 Antibodies 9 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies 13 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 35 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Epitope N-Term
(20), (16), (9), (6), (6), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
(157), (48), (23), (20), (1), (1)
Host Rabbit
(101), (71), (21)
Clonality
Polyclonal
Conjugate This Perforin 1 antibody is un-conjugated
(16), (12), (10), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(95), (83), (44), (41), (37), (37), (36), (34), (30), (6), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN2775294) Images
Blocking Peptide Blocking peptide for this product available: ABIN982195
Sequence SVAGSHSQAA NFAAQKTHQD QYSFSTDTVE CRFYSFHVVH TPPLHPDFKR
Predicted Reactivity Cow: 77%, Dog: 92%, Guinea Pig: 77%, Horse: 85%, Human: 100%, Pig: 100%, Rabbit: 92%, Rat: 77%
Characteristics This is a rabbit polyclonal antibody against PRF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PRF1

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 Antibody (ABIN2775294) Images back to top
Antigen
Alternative Name PRF1 (PRF1 Antibody Abstract)
Background PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the gene encoding PRF1 cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.
Alias Symbols: FLH2, HPLH2, MGC65093, P1, PFP, PFN1
Protein Interaction Partner: KRT31, FBXO6, PRF1, TRIM54, DDX24, GZMB, CALR,
Protein Size: 555
Molecular Weight 59 kDa
Gene ID 5551
NCBI Accession NM_005041, NP_005032
UniProt P14222
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 Antibody (ABIN2775294) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 555 AA

Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 Antibody (ABIN2775294) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Perforin 1 Antibody (ABIN2775294)

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top
Background publications

Okur, Balta, Akarsu, Oner, Patiroglu, Bay, Sayli, Unal, Gurgey: "Clinical and molecular aspects of Turkish familial hemophagocytic lymphohistiocytosis patients with perforin mutations." in: Leukemia research, Vol. 32, Issue 6, pp. 972-5, 2008

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN2775294) back to top
Supplier Images
Western Blotting (WB) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (N-Term) (ABIN2775294) WB Suggested Anti-PRF1 Antibody Titration: 0.2-1 ug/ml
Positive Control:...