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anti-Mouse (Murine) Perforin 1 antibody for Immunofluorescence

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • Cyta
  • FLH2
  • HPLH2
  • LOC443187
  • P1
  • perforin
  • Pfn
  • PFN1
  • Pfp
  • PFP
  • Prf-1
  • prf1
  • PRF1
  • RATCYTA
Reactivity
Human, Mouse (Murine)
163
47
23
18
1
1
Host
Rabbit
110
71
28
Clonality
Polyclonal
Conjugate
This Perforin 1 antibody is un-conjugated
19
13
10
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
91
81
62
60
46
45
45
44
31
9
6
6
3
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2403408
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN411557 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN187562 IF FITC Rat IgG2a AA 402-534 Log in to see CB5-4
0.0010840179 ABIN187239 EIA FACS IHC (fro) IF WB Rat IgG2a AA 189-360 Log in to see KM585 (P1-8) 4
0.0010840179 ABIN3197764 IC IF WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN187561 IHC (fro) IF IHC (p) WB Rat IgG2a Log in to see CB5-4
0.0010840179 ABIN4344818 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344820 ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344822 ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344824 ICC IF IHC IHC (fro) IHC (p) IP WB PE Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344826 ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344828 ICC IF IHC IHC (fro) IHC (p) IP WB Allophycocyanin Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344830 ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344832 ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344834 ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405LS Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344836 ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344838 ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344840 ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344842 ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN4344844 ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
0.0010840179 ABIN2988384 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Perforin 1 antibody for Immunofluorescence

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Application / Reactivity Mouse (Murine) Human
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Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 36 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Human, Mouse (Murine)
(163), (47), (23), (18), (1), (1)
Host Rabbit
(110), (71), (28)
Clonality
Polyclonal
Conjugate This Perforin 1 antibody is un-conjugated
(19), (13), (10), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(91), (81), (62), (60), (46), (45), (45), (44), (31), (9), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling Images
Purification Antigen affinity purification
Immunogen Recombinant protein of human PRF1
Isotype IgG

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRF1 (PRF1 Antibody Abstract)
Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.
Synonyms: PRF1,FLH2,HPLH2,MGC65093,P1,PFN1,PFP
Molecular Weight Observed MW: 61kDa
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Images back to top
Format Liquid
Handling Advice Avoid freeze / thaw cycles.
Storage -20 °C/-80 °C
Storage Comment Store at -20°C (regular) and -80°C (long term).

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Immunofluorescence analysis analysis of HeLa cell using PRF1 antibody. Blue: DAPI for...
Immunohistochemistry (IHC) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Immunohistochemistry of paraffin-embedded human lung cancer using PRF1 antibody at di...
Immunohistochemistry (IHC) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Immunohistochemistry of paraffin-embedded human kidney cancer using PRF1 antibody at ...
Western Blotting (WB) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Western blot analysis of extracts of various cell lines, using PRF1 antibody.