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|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies|
|Conjugate||This TNFRSF12A antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-TNFRSF12A AntibodyTarget Details TNFRSF12A Application Details Handling Images
|Predicted Reactivity||Frog,Mouse (Murine),Rat (Rattus)|
|Characteristics||Cellular Localization: Membrane|
|Purification||Purified by Protein A/G|
|Immunogen||Synthetic peptide near the C-terminus of human Fn14 protein.|
Target Details TNFRSF12AProduct Details anti-TNFRSF12A Antibody Application Details Handling Images back to top
|Alternative Name||Fn14 (TNFRSF12A Antibody Abstract)|
|Background||Fn14/Tweak receptor/CD266/TNFRSF12A, tumor necrosis factor receptor superfamily member 12A, is the receptor for Tweak, a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins.|
|Molecular Weight||14-17 kDa|
|Pathways||Apoptosis, Regulation of Cell Size|
Application DetailsProduct Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Handling Images back to top
|Application Notes||IHC Dilution: 1:100|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue sections in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Images back to top
|Buffer||rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
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