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anti-Human TNFRSF12A antibody for Flow Cytometry

Recommended TNFRSF12A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
  • AI255180
  • C87282
  • CD266
  • Fn14
  • FN14
  • HPIP
  • TWEAK-R
  • TweakR
  • TWEAKR
Epitope
C-Term
13
4
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
95
44
23
Host
Rabbit
50
46
2
Clonality
Monoclonal
Conjugate
This TNFRSF12A antibody is un-conjugated
11
6
5
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
51
47
19
17
14
14
11
11
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN1688720
$ 659.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30686516 ABIN2661016 FACS CyTOF Biotin Mouse IgG2b kappa Log in to see ITEM-4 7
0.30686516 ABIN2663248 FACS PE Mouse IgG2b kappa Log in to see ITEM-4 7
0.30686516 ABIN2663247 FACS PE Mouse IgG1 kappa Log in to see ITEM-1 6
0.30686516 ABIN2666428 BR FACS IF ELISA Mouse IgG1 kappa Log in to see ITEM-1 6
0.30686516 ABIN2664461 FACS Mouse IgG2b kappa Log in to see ITEM-4 8
0.30686516 ABIN2664460 Func FACS IHC Mouse IgG1 kappa Log in to see ITEM-1 6
0.0010666396 ABIN205327 FACS IHC (fro) WB Mouse IgG1 Log in to see ITEM-1
0.0010666396 ABIN2565059 FACS PE Mouse IgG2b, kappa Log in to see ITEM-4
0.0010666396 ABIN1819127 FACS PE Mouse IgG2b Log in to see ITEM-4
0.0010666396 ABIN475685 FACS Biotin Mouse IgG2b, kappa Log in to see ITEM-4
0.0010666396 ABIN475778 FACS PE Mouse IgG1, kappa Log in to see ITEM-1
0.0010666396 ABIN473944 FACS IHC (fro) WB Mouse IgG2b, kappa Log in to see ITEM-4
0.0010666396 ABIN602498 IHC (fro) FACS WB Mouse IgG2b Log in to see
0.0010666396 ABIN1820056 FACS FITC Mouse IgG2b Log in to see ITEM-4
0.0010666396 ABIN2749025 Func IHC (f) FACS WB Mouse IgG2b Log in to see ITEM-4 3
0.0010666396 ABIN2749054 Func IHC (f) FACS WB Mouse IgG2b Log in to see ITEM-4 3
0.0010666396 ABIN1942897 FACS IHC (p) WB Rabbit IgG C-Term Log in to see SP134
0.0010666396 ABIN1820057 IHC (fro) FACS WB Mouse IgG2b Log in to see ITEM-4
0.0010666396 ABIN2749122 Func IHC (f) FACS WB PE Mouse IgG2b Log in to see ITEM-4 3
0.0010666396 ABIN281765 Func FACS IHC (fro) Mouse IgG1 kappa Log in to see ITEM-1

Top referenced anti-TNFRSF12A antibody for Flow Cytometry

Similar anti-TNFRSF12A Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 16 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 46 Antibodies
Functional Studies (Func) 14 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
Epitope C-Term
(13), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(95), (44), (23)
Host Rabbit
(50), (46), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF12A antibody is un-conjugated
(11), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(51), (47), (19), (17), (14), (14), (11), (11), (3), (3), (2), (1), (1), (1), (1)
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Product Details anti-TNFRSF12A Antibody

Target Details TNFRSF12A Application Details Handling Images
Predicted Reactivity Frog,Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Membrane
Purification Purified by Protein A/G
Immunogen Synthetic peptide near the C-terminus of human Fn14 protein.
Clone SP134
Isotype IgG

Target Details TNFRSF12A

Product Details anti-TNFRSF12A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Fn14 (TNFRSF12A Antibody Abstract)
Background Fn14/Tweak receptor/CD266/TNFRSF12A, tumor necrosis factor receptor superfamily member 12A, is the receptor for Tweak, a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins.
Molecular Weight 14-17 kDa
Gene ID 51330
Pathways Apoptosis

Application Details

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Handling Images back to top
Application Notes IHC Dilution: 1:100
Comment

Positive Control:
IHC (p): Renal cell carcinoma, kidney, lung adenocarcinoma
WB: Calu3 cell lysate
FC: HT29 Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue sections in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) Flow cytometric analysis of rabbit anti-Fn14 (SP134) antibody in HT29 (green) compare...
Western Blotting (WB) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 11)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 12)
Immunohistochemistry (IHC) image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (C-Term) (ABIN1688720) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 13)