anti-Human Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Microtubule-Associated Protein 1 Light Chain 3 beta Antibody (supplied by: Log in to see )

Antigen
Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Epitope
AA 1-100, N-Term
85
50
23
21
21
17
10
10
9
8
8
6
6
5
5
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
267
157
110
32
27
27
26
25
12
12
6
6
1
Host
Rabbit
250
20
1
Clonality
Monoclonal
Conjugate
Un-conjugated
18
15
15
14
13
13
6
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
158
121
90
76
72
67
61
37
26
10
8
6
6
3
2
1
1
1
Supplier
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Catalog No. ABIN4330478
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.850952 ABIN1449627 EIA IHC (p) WB Rabbit Ig AA 88-118, C-Term Log in to see Polyclonal 7
13.850952 ABIN153145 EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit N-Term Log in to see Polyclonal 163
13.850952 ABIN250638 ELISA ICC IF IHC IHC (fro) IHC (p) IP PLA SimWes WB Rabbit N-Term, AA 1-100 Log in to see Polyclonal 562
13.850952 ABIN681583 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal 1
1 ABIN250639 ELISA ICC IF IHC (fro) IHC (p) WB Biotin Rabbit N-Term, AA 1-100 Log in to see Polyclonal 4
1 ABIN250640 ELISA IHC (fro) IHC (p) WB HRP Rabbit N-Term, AA 1-100 Log in to see Polyclonal 9
1 ABIN269557 IHC (fro) IHC (p) WB HRP Rabbit N-Term Log in to see Polyclonal 2
1 ABIN5555711 EIA IF IHC (p) WB Rabbit Ig Fraction AA 1-30, N-Term Log in to see Polyclonal
1 ABIN2854667 IHC (p) WB Rabbit IgG full length Log in to see Polyclonal
1 ABIN4330470 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 1-100, N-Term Log in to see Polyclonal 2
1 ABIN4330466 FACS ICC IF IHC (fro) IHC (p) FITC Rabbit N-Term Log in to see Polyclonal 1
1 ABIN4330471 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 1-100, N-Term Log in to see Polyclonal 1
1 ABIN269556 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit N-Term Log in to see Polyclonal 4
1 ABIN406993 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit N-Term, AA 1-100 Log in to see Polyclonal 2
1 ABIN4330463 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 1-100, N-Term Log in to see Polyclonal
1 ABIN4330484 ICC IF IHC IHC (p) WB DyLight 488 Rabbit IgG AA 1-100, N-Term Log in to see 1251A
1 ABIN4330487 ICC IF IHC IHC (p) WB DyLight 680 Rabbit IgG AA 1-100, N-Term Log in to see 1251A
1 ABIN4330486 ICC IF IHC IHC (p) WB DyLight 650 Rabbit IgG AA 1-100, N-Term Log in to see 1251A
1 ABIN4330491 ICC IF IHC IHC (p) WB PerCP Rabbit IgG AA 1-100, N-Term Log in to see 1251A
1 ABIN4330492 ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit IgG AA 1-100, N-Term Log in to see 1251A

Top referenced anti-Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Microtubule-Associated Protein 1 Light Chain 3 beta Antibodies

Application / Reactivity Mouse (Murine) Human
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 34 Antibodies 119 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 72 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 83 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 10 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Simple Western (SimWes) 6 Antibodies
Western Blotting (WB) 131 Antibodies
Dot Blot (DB) 76 Antibodies

General

Antigen Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies
Epitope AA 1-100, N-Term
(85), (50), (23), (21), (21), (17), (10), (10), (9), (8), (8), (6), (6), (5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(267), (157), (110), (32), (27), (27), (26), (25), (12), (12), (6), (6), (1)
Host Rabbit
(250), (20), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(18), (15), (15), (14), (13), (13), (6), (5), (5), (5), (5), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(158), (121), (90), (76), (72), (67), (61), (37), (26), (10), (8), (6), (6), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product details

Target details Application Details Handling References Images
Specificity Specific for LC3B.
Purification Protein G purified
Immunogen A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8].
Clone 1251A
Isotype IgG

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Research Area Cytoskeleton, Autophagy, Cancer
Pathways Autophagy

Application Details

Product details Target details Handling References Images back to top
Application Notes Western Blot 1.0 - 2.0 μg/mL, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 10-20 μg/mL, Immunoprecipitation 2-10 μg, Immunohistochemistry-Paraffin 1:100-1:500, Flow (Intracellular) 5 μL per 1 million cellsIn Western blot this LC3B recombinant monoclonal antibody detects both LC3I and LC3II with Chloroquine treatment. With ICC autophagosome staining was observed after treatment with Chloroquine.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

Product details Target details Application Details Handling Images back to top
Product cited in:

Wechman, Rao, Cheng, Gomez-Gutierrez, McMasters, Zhou: "Development of an Oncolytic Adenovirus with Enhanced Spread Ability through Repeated UV Irradiation and Cancer Selection." in: Viruses, Vol. 8, Issue 6, 2016 Further details: Western Blotting

Verwey, Nolte, Joubert, Theron: "Autophagy induced by a sulphamoylated estrone analogue contributes to its cytotoxic effect on breast cancer cells." in: Cancer cell international, Vol. 16, pp. 91, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] -...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - IHC anal...
Immunoprecipitation (IP) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunoprecipitation: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - Western blot analy...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Western Blot: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - Western Blot image of mon...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] -...