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|Application / Reactivity||Zebrafish||Rat (Rattus)|
|Electron Microscopy (EM)||1 Antibodies||1 Antibodies|
|ELISA||12 Antibodies||20 Antibodies|
|Flow Cytometry (FACS)||12 Antibodies||16 Antibodies|
|Immunocytochemistry (ICC)||23 Antibodies||48 Antibodies|
|Immunofluorescence (IF)||22 Antibodies||59 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||24 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Simple Western (SimWes)||2 Antibodies|
|Western Blotting (WB)||24 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunoelectron Microscopy (IEM)||1 Antibodies|
|Antigen||Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Zebrafish Alternatives|
Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
|20 references available|
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|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to the N-terminal region of the human LC3B protein. [UniProt# Q9GZQ8]|
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|Alternative Name||LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)|
|Background||Gene Symbol: MAP1LC3B|
|Research Area||Cytoskeleton, Autophagy, Cancer|
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|Application Notes||Western Blot 1:1000, Simple Western 1:100, Flow Cytometry 1:200, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 0.1-2 μg/mL, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:400, Electron MicroscopyThis LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for LC3B Antibody Fluorescent Staining
1. After washing sample(s) in PBS, fix for 20 minutes at room temperature.
. Wash sample(s) in PBS 3x 3 minutes, each.
. Block sample(s) with 5 % NGS (keep Triton X-100 at 0.1 %) for 60 minutes at room temperature
. Prepare primary anti-LC3B solution during this time at a 1:5,000 dilution with 1 %NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the primary Ab overnight at 4C in a humidified containerNote: If thick tissue samples, Ab must be prepared in 0.5 % Triton X-100/PBSDay
. Wash sample(s) in PBS 3x 5 minutes with wells on. Prepare Alexa-fluor antibodies (Goat anti-Rabbit IgG H+L) as secondary Ab with 1 % NGS (keep Triton X-100 at 0.1 %).
. Incubate sample(s) in the appropriate secondary Ab for 1 hour in a dark container.
. Rinse the sample(s) in PBS 4x 10 minutes, each.
. Place one drop of Vectashield onto the slide and cover with a coverslip
|Restrictions||For Research Use only|
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Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
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|Product cited in:||
Liu, Weng, Lin, Tang, Chen, Liang, Ku, Lin: "Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells." in: Scientific reports, Vol. 7, pp. 41437, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Kristensen, Oppermann, Rauen, Hartmann-Petersen, Thirstrup: "Polyglutamine expansion of ataxin-3 alters its degree of ubiquitination and phosphorylation at specific sites." in: Neurochemistry international, Vol. 105, pp. 42-50, 2017
Lu, Bradley, McCoski, Gonzalez, Ealy, Johnson: "Reduced skeletal muscle fiber size following caloric restriction is associated with calpain-mediated proteolysis and attenuation of IGF-1 signaling." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 312, Issue 5, pp. R806-R815, 2017
Myeku, Clelland, Emrani, Kukushkin, Yu, Goldberg, Duff: "Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling." in: Nature medicine, Vol. 22, Issue 1, pp. 46-53, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Santanam, Banach-Petrosky, Abate-Shen, Shen, White, DiPaola: "Atg7 cooperates with Pten loss to drive prostate cancer tumor growth." in: Genes & development, Vol. 30, Issue 4, pp. 399-407, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry
Bauckman, Mysorekar: "Ferritinophagy drives uropathogenic Escherichia coli persistence in bladder epithelial cells." in: Autophagy, Vol. 12, Issue 5, pp. 850-63, 2016 (Sample species: Human). Further details: Western Blotting
Alán, Špaček, Pajuelo Reguera, Jabůrek, Ježek: "Mitochondrial nucleoid clusters protect newly synthesized mtDNA during Doxorubicin- and Ethidium Bromide-induced mitochondrial stress." in: Toxicology and applied pharmacology, Vol. 302, pp. 31-40, 2016 (Sample species: Human). Further details: Western Blotting
Lee, Du, Stitham, Atteya, Lee, Xiang, Wang, Jin, Leslie, Spollett, Srivastava, Mannam, Ostriker, Martin, Tang, Hwa: "Inducing mitophagy in diabetic platelets protects against severe oxidative stress." in: EMBO molecular medicine, Vol. 8, Issue 7, pp. 779-95, 2016 (Sample species: Human). Further details: Flow Cytometry
Cemma, Grinstein, Brumell: "Autophagy proteins are not universally required for phagosome maturation." in: Autophagy, Vol. 12, Issue 9, pp. 1440-6, 2016 Further details: Western Blotting
Shui, Liu, Zhu, Wang: "Exogenous hydrogen sulfide attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy in mice." in: Canadian journal of physiology and pharmacology, pp. 1-6, 2016
Sousa, Biancur, Wang, Halbrook, Sherman, Zhang, Kremer, Hwang, Witkiewicz, Ying, Asara, Evans, Cantley, Lyssiotis, Kimmelman: "Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion." in: Nature, Vol. 536, Issue 7617, pp. 479-83, 2016 Further details: Western Blotting
Qi, Man, Malireddi, Karki, Lupfer, Gurung, Neale, Guy, Lamkanfi, Kanneganti: "Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection." in: The Journal of experimental medicine, Vol. 213, Issue 10, pp. 2081-97, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Zhou, Wen, Cheng, Ma, Li, Ren, Sheng, Dong, Lu, Hu, Wang: "Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-κB signal pathway." in: Oncoimmunology, Vol. 5, Issue 7, pp. e1180485, 2016 Further details: Flow Cytometry
Wang, Han, Sun, Feng et al.: "Cerebral ischemic post‑conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation ..." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4162-4172, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence
Gschweitl, Ulbricht, Barnes, Enchev, Stoffel-Studer, Meyer-Schaller, Huotari, Yamauchi, Greber, Helenius, Peter: "A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes." in: eLife, Vol. 5, pp. e13841, 2016 (Sample species: Human). Further details: Western Blotting
Yu, Xie, Cao, Yang, Yang, Lotze, Zeh, Kang, Tang: "The ferroptosis inducer erastin enhances sensitivity of acute myeloid leukemia cells to chemotherapeutic agents." in: Molecular & cellular oncology, Vol. 2, Issue 4, pp. e1054549, 2016 Method employed by authors: Western Blotting (WB)
Dickinson, Alevy, Malvin, Patel, Gunsten, Holtzman, Stappenbeck, Brody: "IL13 activates autophagy to regulate secretion in airway epithelial cells." in: Autophagy, Vol. 12, Issue 2, pp. 397-409, 2016 Method employed by authors: Western Blotting (WB)
Boman, Svensson, Boxer, Rojas, Seeley, Karydas, Miller, Kågedal, Svenningsson: "Distinct Lysosomal Network Protein Profiles in Parkinsonian Syndrome Cerebrospinal Fluid." in: Journal of Parkinson's disease, Vol. 6, Issue 2, pp. 307-15, 2016 Method employed by authors: Western Blotting (WB)
Vodicka, Chase, Iuliano, Tousley, Valentine, Sapp, Kegel-Gleason, Sena-Esteves, Aronin, DiFiglia: "Autophagy Activation by Transcription Factor EB (TFEB) in Striatum of HDQ175/Q7 Mice." in: Journal of Huntington's disease, Vol. 5, Issue 3, pp. 249-260, 2016
Schläfli, Adams, Galván, Gugger, Savic, Bubendorf, Schmid, Becker, Tschan, Langer, Berezowska: "Prognostic value of the autophagy markers LC3 and p62/SQSTM1 in early-stage non-small cell lung cancer." in: Oncotarget, Vol. 7, Issue 26, pp. 39544-39555, 2016 Method employed by authors: Immunohistochemistry (IHC), Western Blotting (WB) (Sample species: Human).
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