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ARAF antibody (C-Term)

Name: ARAF antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2855039
Price: 609.18 USD
Availability: InStock

ARAF Reactivity: Human, Mouse WB, IHC (p), IF, ICC Host: Rabbit Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: Hela 7. (ARAF antibody (C-Term))

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF, antibody at 1:200 dilution. (ARAF antibody (C-Term))

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7. (ARAF antibody (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF, antibody at 1:500 dilution. (ARAF antibody (C-Term))

IHC

4 images

Catalog No. ABIN2855039

Datasheet as PDF

Target See all ARAF Antibodies

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
Binding Specificity
19

18

15

15

9

8

7

7

6

6

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
126

50

32

4

4

4

3

2

2

2

1
Human, Mouse
Host
121

5
Rabbit
Compatible Secondaries
Clonality
111

15
Polyclonal
Conjugate
49

9

7

6

6

6

6

6

6

6

3

3

3

3

3

2

2
This ARAF antibody is un-conjugated
Application
77

43

40

39

20

15

11

9

4

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity

Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio), Cow (Bovine)

Cross-Reactivity (Details)

Pig (99 %), Rat (99 %), Zebrafish (85 %), Bovine (99 %)

Characteristics

Rabbit Polyclonal antibody to A-RAF (v-raf murine sarcoma 3611 viral oncogene homolog)
A-RAF antibody [C1C3]

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.

Isotype

IgG

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anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 151-250) antibody
ARAF Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3E2 unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibody
ARAF Reactivity: Human, Mouse WB, IF (cc), IHC (p) Host: Rabbit Monoclonal 24D1 unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) antibody
ARAF Reactivity: Human WB, IF, PLA Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 160-310) antibody
ARAF Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pSer299) antibody
ARAF Reactivity: Human WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr301), (pTyr302) antibody
ARAF Reactivity: Human, Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibody
ARAF Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 256-286) antibody
ARAF Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB03325 unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 371-606) antibody
ARAF Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibody
ARAF Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions

For Research Use only
Format

Liquid

Concentration

0.85 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name

A-RAF (ARAF Products)

Synonyms

zgc:92074 antibody, wu:fk45h07 antibody, ARAF antibody, a-raf antibody, araf antibody, araf1 antibody, pks2 antibody, rafa1 antibody, A-RAF antibody, ARAF1 antibody, PKS2 antibody, RAFA1 antibody, 1200013E08Rik antibody, A-Raf antibody, AW495444 antibody, Araf1 antibody, A-Raf proto-oncogene, serine/threonine kinase antibody, A-Raf proto-oncogene, serine/threonine kinase L homeolog antibody, A-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Araf proto-oncogene, serine/threonine kinase antibody, araf antibody, ARAF antibody, araf.L antibody, araf.S antibody, Araf antibody

Background

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.

Molecular Weight

68 kDa

Gene ID

369

Pathways

MAPK Signaling, RTK Signaling, Hepatitis C