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HIF1A antibody (AA 329-530)

Name: HIF1A antibody (AA 329-530)
SKU: ABIN534065
Price: 1050 USD
Availability: InStock

HIF1A Reactivity: Human IHC, IF, IP, ICC, IHC (p), IHC (fro) Host: Mouse Monoclonal ESEE122 unconjugated

Staining of HIF1A in hypoxia-induced human placenta using HIF1A monoclonal antibody, clone ESEE122 . (HIF1A antibody (AA 329-530))

IHC

Negative control stain of human placenta (from sea level) using mouse IgG at 1 : 100. (HIF1A antibody (AA 329-530))

Staining of HIF1A (red dye 568) in a cultured Raw 264. (HIF1A antibody (AA 329-530))

Detection of HIF1A (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using HIF1A monoclonal antibody, clone ESEE122 . (HIF1A antibody (AA 329-530))

4 images

(6 references)

Catalog No. ABIN534065

Datasheet as PDF

Target See all HIF1A Antibodies

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
Binding Specificity
20

19

16

16

12

7

7

7

6

6

5

4

4

4

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 329-530
Reactivity
166

86

81

24

23

20

14

12

9

8

7

6

6

5

4

2

2

2

2

2

1

1

1

1

1

1

1
Human
Host
159

52

6
Mouse
Compatible Secondaries
Clonality
165

52
Monoclonal
Conjugate
114

29

18

8

6

4

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HIF1A antibody is un-conjugated
Application
188

89

76

56

51

39

39

26

26

26

12

8

7

7

3

3

3

2

1

1

1

1
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose

Mouse monoclonal antibody raised against partial recombinant HIF1A.

Specificity

This antibody is specific to HIF-1 alpha.

Cross-Reactivity

Cow, Dog, Human, Mouse, Rat

Immunogen

Recombinant protein corresponding to amino acids 329-530 of human HIF1A.

Clone

ESEE122

Isotype

IgG1

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HIF1A Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

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HIF1A Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB14548 unconjugated

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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 216-336) antibody
HIF1A Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody
HIF1A Reactivity: Human, Mouse WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 728-757) antibody
HIF1A Reactivity: Human, Mouse WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732) antibody
HIF1A Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Immunocytochemistry (1:100-1:5000)
Immunofluorescence (1:100-1:5000)
Immunohistochemistry (Frozen sections) (1:100-1:5000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:5000)
The optimal working dilution should

Restrictions

For Research Use only
Format

Liquid

Buffer

In buffer containing 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.
Cecchi, Sestili, Prosperini, Cecchetto, Vicini, Viel, Muciaccia: "Markers of mechanical asphyxia: immunohistochemical study on autoptic lung tissues." in: International journal of legal medicine, Vol. 128, Issue 1, pp. 117-25, (2014) (PubMed).

Gomes, Rodrigues, Phadke, Butcher, Starling, Chen, Chang, Hernandez-Alcoceba, Newman, Stone, Tong: "Antitumor activity of an oncolytic adenoviral-CD40 ligand (CD154) transgene construct in human breast cancer cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 4, pp. 1317-25, (2009) (PubMed).

Kubo, Sugita, Shimose, Matsuo, Arihiro, Ochi: "Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours." in: The Journal of bone and joint surgery. British volume, Vol. 90, Issue 3, pp. 364-70, (2008) (PubMed).

Piovan, Tosello, Indraccolo, Masiero, Persano, Esposito, Zamarchi, Ponzoni, Chieco-Bianchi, Dalla-Favera, Amadori: "Differential regulation of hypoxia-induced CXCR4 triggering during B-cell development and lymphomagenesis." in: Cancer research, Vol. 67, Issue 18, pp. 8605-14, (2007) (PubMed).

Khan, Michalopoulos, Stolz: "Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation." in: The American journal of pathology, Vol. 169, Issue 4, pp. 1251-69, (2006) (PubMed).

Choke, Cockerill, Dawson, Chung, Griffiths, Wilson, Loftus, Thompson: "Hypoxia at the site of abdominal aortic aneurysm rupture is not associated with increased lactate." in: Annals of the New York Academy of Sciences, Vol. 1085, pp. 306-10, (2006) (PubMed).
Target

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name

HIF1A / HIF1 alpha (HIF1A Products)

Synonyms

hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody

Gene ID

3091

Pathways

Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect