SEMA7A Protein (AA 45-646) (His tag)
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- Target See all SEMA7A Proteins
- SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 45-646
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SEMA7A protein is labelled with His tag.
- Purpose
- Mouse SEMA7A Protein
- Sequence
- Gln45-Ala646
- Characteristics
- Recombinant Mouse SEMA7A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ala646.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))
- Alternative Name
- SEMA7A (SEMA7A Products)
- Synonyms
- 2900057C09Rik Protein, CDw108 Protein, H-Sema-L Protein, M-Sema-L Protein, Semal Protein, CD108 Protein, H-SEMA-K1 Protein, JMH Protein, SEMAK1 Protein, SEMAL Protein, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A Protein, semaphorin 7A (John Milton Hagen blood group) Protein, Sema7a Protein, SEMA7A Protein
- Background
- Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.
- Molecular Weight
- 69.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9QUR8
- Pathways
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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