SEMA7A Protein (AA 45-648) (His tag)

Catalog No. ABIN7275564

Product Details

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Protein Type: Recombinant

Protein Characteristics: AA 45-648

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SEMA7A protein is labelled with His tag.

Purpose: Human SEMA7A Protein

Sequence: Gln45-Ala648

Characteristics: Recombinant Human SEMA7A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ala648.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Alternative Name: SEMA7A (SEMA7A Products)

Synonyms: 2900057C09Rik Protein, CDw108 Protein, H-Sema-L Protein, M-Sema-L Protein, Semal Protein, CD108 Protein, H-SEMA-K1 Protein, JMH Protein, SEMAK1 Protein, SEMAL Protein, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A Protein, semaphorin 7A (John Milton Hagen blood group) Protein, Sema7a Protein, SEMA7A Protein

Background: Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.

Molecular Weight: 69.5 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size