Kallikrein 5 Protein (KLK5) (AA 23-293) (His tag)
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- Target See all Kallikrein 5 (KLK5) Proteins
- Kallikrein 5 (KLK5)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-293
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Kallikrein 5 protein is labelled with His tag.
- Purpose
- Human Kallikrein 5/KLK5 Protein
- Sequence
- Val23-Ser293
- Characteristics
- Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val23-Ser293.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Kallikrein 5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl ( pH 5.5).
- Buffer
- Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Kallikrein 5 (KLK5)
- Alternative Name
- Kallikrein 5 (KLK5 Products)
- Synonyms
- KLK-L2 Protein, KLKL2 Protein, SCTE Protein, 1110030O19Rik Protein, KAL Protein, KALA Protein, Klk1 Protein, Klk5 Protein, RATKALA Protein, kallikrein related peptidase 5 Protein, kallikrein related-peptidase 5 Protein, kallikrein 5-like Protein, KLK5 Protein, Klk5 Protein, Klk5l Protein
- Background
- The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
- Molecular Weight
- 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9Y337
- Pathways
- Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling
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