anti-Human Glutaminase antibody for Western Blotting

Recommended Glutaminase Antibody (supplied by: Log in to see )

Antigen
Glutaminase (GLS) Antibodies
  • Glut
  • RATGLUT
  • gls
  • si:dz87i4.2
  • wu:fa97e05
  • GA
  • PAG
  • AAD20
  • GAC
  • GAM
  • GLS1
  • KGA
  • AI314027
  • 6330442B14
  • B230365M23Rik
  • CG8772
  • CG8872
  • Dmel\\CG42708
  • Dmel_CG8772
  • A330074B06Rik
  • AI195532
  • GLS
  • Lga
  • BA3155
  • glutaminase
  • glutaminase b
  • Glutaminase
  • Putative glutaminase 3
  • GLutamiNAse
  • glutaminase 2 (liver, mitochondrial)
  • glutaminase kidney isoform, mitochondrial
  • Gls
  • GLS
  • gls
  • glsb
  • glna-3
  • glna-2
  • Gls2
  • glsA2
  • glsA-2
  • Arnit_3113
  • Celal_2913
  • Celly_0289
  • Weevi_2101
  • Mesop_4683
  • LOC100069617
Epitope
C-Term
20
11
9
8
6
2
2
1
Reactivity
Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
90
60
31
4
1
1
1
1
1
1
Host
Rabbit
63
34
Clonality
Polyclonal
Conjugate
This Glutaminase antibody is un-conjugated
6
6
6
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
79
43
41
33
27
24
13
12
5
2
1
Options
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Catalog No. ABIN2786054
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.210425 ABIN652757 FACS IF IHC (p) WB Rabbit Ig AA 516-545, C-Term Log in to see Polyclonal 1
3.210425 ABIN4314688 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.210425 ABIN1872837 IHC IP WB Rabbit IgG Log in to see Polyclonal
3.210425 ABIN561056 IF ELISA WB Mouse IgG2a kappa AA 580-669, partial Log in to see 5C4 4
3.210425 ABIN396559 IF WB Mouse IgG2a kappa AA 580-670 Log in to see 5C4
3.210425 ABIN4891794 WB Rabbit Log in to see Polyclonal
3.210425 ABIN4274400 ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 3211
3.210425 ABIN5539364 IF IHC (p) WB Rabbit Ig Fraction AA 516-545, C-Term Log in to see Polyclonal
3.210425 ABIN634050 WB Rabbit Middle Region Log in to see Polyclonal
3.210425 ABIN4314689 FACS ICC IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
3.210425 ABIN2433089 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.210425 ABIN1868177 ICC IHC IP WB Rabbit IgG AA 221-533 Log in to see Polyclonal
3.210425 ABIN5664498 IHC WB Rabbit Log in to see Polyclonal
1 ABIN469690 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2473803 IF WB Mouse IgG2a AA 580-670 Log in to see 5C4 1
1 ABIN561055 ELISA WB Mouse AA 580-669, partial Log in to see Polyclonal 1
1 ABIN1898865 ELISA FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1898869 ELISA FACS IF IHC WB PE Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1898868 ELISA FACS IF IHC WB APC Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1898866 ELISA FACS IF IHC WB Biotin Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Glutaminase (GLS) Antibodies
Epitope C-Term
(20), (11), (9), (8), (6), (2), (2), (1)
Reactivity Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(90), (60), (31), (4), (1), (1), (1), (1), (1), (1)
Host Rabbit
(63), (34)
Clonality
Polyclonal
Conjugate This Glutaminase antibody is un-conjugated
(6), (6), (6), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(79), (43), (41), (33), (27), (24), (13), (12), (5), (2), (1)
Pubmed 1 reference available
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Product Details anti-Glutaminase Antibody

Target Details Glutaminase Application Details Handling References for anti-Glutaminase antibody (ABIN2786054) Images
Blocking Peptide Blocking peptide for this product available: ABIN977471
Sequence VNPFPKDRWN NTPMDEALHF GHHDVFKILQ EYQVQYTPQG DSDNGKENQT
Predicted Reactivity Dog: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against GLS. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the c terminal region of human GLS

Target Details Glutaminase

Product Details anti-Glutaminase Antibody Application Details Handling References for anti-Glutaminase antibody (ABIN2786054) Images back to top
Antigen
Alternative Name GLS (GLS Antibody Abstract)
Background Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances
Alias Symbols: AAD20, DKFZp686O15119, FLJ10358, GLS1, KIAA0838, GAC, GAM, KGA
Protein Interaction Partner: UBC, PEPD, MVD, GNS, MYL12A, GDA, EIF5A, CAPN1, ATF2, ATXN10, CUL5, TAX1BP3, PARD6A, SNTA1,
Protein Size: 669
Molecular Weight 73 kDa
Gene ID 2744
NCBI Accession NM_014905, NP_055720
UniProt O94925
Pathways Feeding Behaviour, Dicarboxylic Acid Transport

Application Details

Product Details anti-Glutaminase Antibody Target Details Glutaminase Handling References for anti-Glutaminase antibody (ABIN2786054) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 669 AA

Restrictions For Research Use only

Handling

Product Details anti-Glutaminase Antibody Target Details Glutaminase Application Details References for anti-Glutaminase antibody (ABIN2786054) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Glutaminase antibody (ABIN2786054)

Product Details anti-Glutaminase Antibody Target Details Glutaminase Application Details Handling Images back to top
Product cited in:

Sahai: "Glutaminase in human platelets." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 127, Issue 2, pp. 197-203, 1983

Images

Product Details anti-Glutaminase Antibody Target Details Glutaminase Application Details Handling References for anti-Glutaminase antibody (ABIN2786054) back to top
Supplier Images
Western Blotting (WB) image for anti-Glutaminase (GLS) (C-Term) antibody (ABIN2786054) WB Suggested Anti-GLS Antibody Titration: 0.2-1 ug/ml
Positive Control: ...
Western Blotting (WB) image for anti-Glutaminase (GLS) (C-Term) antibody (ABIN2786054) anti-Glutaminase (GLS) (C-Term) antibody (Image 2)
Western Blotting (WB) image for anti-Glutaminase (GLS) (C-Term) antibody (ABIN2786054) WB Suggested Anti-GLS
Antibody Titration: 0.2-1 µg/mL
Positive Control:...