You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CISH antibody for Immunocytochemistry

Recommended CISH Antibody (supplied by: Log in to see )

Antigen
Cytokine Inducible SH2-Containing Protein (CISH) Antibodies
  • CISH
  • cis
  • cish.a
  • ptk9l
  • twf1l
  • wu:fk57g04
  • zgc:154062
  • zgc:91817
  • g18
  • socs
  • cis-1
  • cish
  • BACTS2
  • CIS
  • CIS-1
  • G18
  • SOCS
  • AI385595
  • CIS1
  • Cis
  • F17
  • F23
  • cytokine inducible SH2-containing protein
  • cytokine-inducible SH2-containing protein
  • Cytokine-inducible SH2-containing protein
  • CISH
  • cish
  • Cish
Epitope
AA 241-258, C-Term
6
5
4
2
2
1
1
1
1
Reactivity
Human, Rat (Rattus)
27
16
14
4
3
3
3
2
2
2
1
Host
Rabbit
28
2
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
27
9
9
8
7
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN3044172
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2854656 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN440988 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2619282 ICC IHC (p) WB Rabbit IgG AA 241-258 Log in to see Polyclonal
1 ABIN4298809 ICC IF IHC IHC (p) Rabbit Log in to see Polyclonal
1 ABIN4994413 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN3030694 ICC IHC (p) WB Rabbit IgG AA 241-258 Log in to see Polyclonal
1 ABIN5013433 ICC IHC IP WB Rabbit IgG AA 1-258 Log in to see Polyclonal
1 ABIN2137676 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-CISH Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 9 Antibodies
Immunofluorescence (IF) 3 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 9 Antibodies
Western Blotting (WB) 13 Antibodies 25 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Cytokine Inducible SH2-Containing Protein (CISH) Antibodies
Epitope AA 241-258, C-Term
(6), (5), (4), (2), (2), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(27), (16), (14), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(28), (2)
Clonality
Polyclonal
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(27), (9), (9), (8), (7), (3), (2), (1)
Supplier Log in to see

Product Details anti-CISH Antibody

Target Details CISH Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Cytokine-inducible SH2-containing protein(CISH) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Sequence LPLPRRMADY LRQYPFQL
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Cytokine-inducible SH2-containing protein(CISH) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: cytokine inducible SH2-containing protein
Protein Name: Cytokine-inducible SH2-containing protein(CIS)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human CISH(241-258aa LPLPRRMADYLRQYPFQL), identical to the related rat and mouse sequences.
Isotype IgG

Target Details CISH

Product Details anti-CISH Antibody Application Details Handling Images back to top
Antigen
Alternative Name CISH (CISH Antibody Abstract)
Background CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

Synonyms: BACTS2 antibody|cis 1 antibody|CIS antibody|CIS-1 antibody|CIS1 antibody|CISH antibody|CISH_HUMAN antibody|Cytokine inducible inhibitor of signaling type 1B antibody|Cytokine inducible SH2 containing protein antibody|Cytokine-inducible SH2-containing protein antibody|G18 antibody|Protein G18 antibody|SOCS antibody|Suppressor of cytokine signaling antibody
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Response to Growth Hormone Stimulus

Application Details

Product Details anti-CISH Antibody Target Details CISH Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CISH Antibody Target Details CISH Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-CISH Antibody Target Details CISH Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CISH antibody (Cytokine Inducible SH2-Containing Protein) (AA 241-258) (ABIN3044172) anti-Cytokine Inducible SH2-Containing Protein (CISH) (AA 241-258), (C-Term) antibody
Immunohistochemistry (IHC) image for anti-CISH antibody (Cytokine Inducible SH2-Containing Protein) (AA 241-258) (ABIN3044172) Anti-CISH antibody, IHC(P) IHC(P): Rat Kidney Tissue
Immunohistochemistry (IHC) image for anti-CISH antibody (Cytokine Inducible SH2-Containing Protein) (AA 241-258) (ABIN3044172) Anti-CISH antibody, IHC(P) IHC(P): Human Lung Cancer Tissue