anti-Human IFNB1 antibody for Enzyme Immunoassay

Recommended IFNB1 Antibody (supplied by: Log in to see )

Antigen
Interferon, beta 1, Fibroblast (IFNB1) Antibodies
  • IFN-R
  • IFN-R-2
  • IFNBR
  • IFN-beta
  • IFNB
  • Ifb
  • Ifnb
  • IFB
  • IFF
  • IFNb
  • interferon, beta 1, fibroblast
  • interferon (alpha, beta and omega) receptor 2
  • interferon beta 1, fibroblast
  • IFNB1
  • LOC100342931
  • IFNAR2
  • Ifnb1
  • IFNB
Epitope
N-Term
21
17
16
12
12
9
8
8
7
6
6
5
4
3
3
3
2
2
2
1
1
1
Reactivity
Human
292
39
18
15
11
10
7
6
5
3
1
1
Host
Rabbit
186
165
8
6
6
4
3
Clonality
Polyclonal
Conjugate
This IFNB1 antibody is un-conjugated
32
25
12
8
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
316
263
96
51
29
24
22
17
16
15
14
10
10
8
5
4
2
2
2
2
1
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Catalog No. ABIN453696
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554858 EIA IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN181769 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN181768 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN116507 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN181770 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2723331 EIA Rabbit IgG Log in to see Polyclonal

General

Antigen Interferon, beta 1, Fibroblast (IFNB1) Antibodies
Epitope N-Term
(21), (17), (16), (12), (12), (9), (8), (8), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human
(292), (39), (18), (15), (11), (10), (7), (6), (5), (3), (1), (1)
Host Rabbit
(186), (165), (8), (6), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IFNB1 antibody is un-conjugated
(32), (25), (12), (8), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(316), (263), (96), (51), (29), (24), (22), (17), (16), (15), (14), (10), (10), (8), (5), (4), (2), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-IFNB1 Antibody

Target Details IFNB1 Application Details Handling References for anti-IFNB1 antibody (ABIN453696) Images
Specificity This antibody reacts to IFNB1.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human Interferon beta 1/IFNB1.

Target Details IFNB1

Product Details anti-IFNB1 Antibody Application Details Handling References for anti-IFNB1 antibody (ABIN453696) Images back to top
Antigen
Alternative Name IFNB / Interferon beta (IFNB1 Antibody Abstract)
Background The mammalian type I Inteferons (IFN1) are produced in response to viral infection and other inducers. They are divided into alpha and beta subtypes leukocytes and fibroblasts reactivity. The human IFN alphas are encoded by a family of at least 15 different genes, while IFN beta is the unique member of its subtype. There is approximately 50 % amino acid homology between the alpha and beta subtypes. Both IFN subtypes are pleiotropic cytokines and have a similar range of biological activities. Differences between alpha subtypes, and between IFN alpha and betas, are in potency and cell type specific activities. In particular, IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2. IFNB1 has antiviral, antibacterial and anticancer activities.Synonyms: Fibroblast interferon, IFB, IFN-beta, IFNB1
Gene ID 3456
NCBI Accession NP_002167
UniProt P01574
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy

Application Details

Product Details anti-IFNB1 Antibody Target Details IFNB1 Handling References for anti-IFNB1 antibody (ABIN453696) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IFNB1 Antibody Target Details IFNB1 Application Details References for anti-IFNB1 antibody (ABIN453696) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IFNB1 antibody (ABIN453696)

Product Details anti-IFNB1 Antibody Target Details IFNB1 Application Details Handling Images back to top
Background publications

Lienenklaus, Cornitescu, Zietara, Łyszkiewicz, Gekara, Jabłónska, Edenhofer, Rajewsky, Bruder, Hafner, Staeheli, Weiss: "Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 5, pp. 3229-36, 2009

Eto, Takahashi: "Enhanced inhibition of hepatitis B virus production by asialoglycoprotein receptor-directed interferon." in: Nature medicine, Vol. 5, Issue 5, pp. 577-81, 1999

Images

Product Details anti-IFNB1 Antibody Target Details IFNB1 Application Details Handling References for anti-IFNB1 antibody (ABIN453696) back to top
Supplier Images
Western Blotting (WB) image for anti-Interferon, beta 1, Fibroblast (IFNB1) (N-Term) antibody (ABIN453696) Western blot analysis of IFNB1 Antibody (N-term) in HepG2 cell line lysates (35ug/lan...